Hyper-stimulation of Pyrococcus furiosus CRISPR DNA uptake by a self-transmissible plasmid.
CRISPR
Conjugative plasmid
Mobile genetic element
Primed adaptation
Pyrococcus furiosus
Journal
Extremophiles : life under extreme conditions
ISSN: 1433-4909
Titre abrégé: Extremophiles
Pays: Germany
ID NLM: 9706854
Informations de publication
Date de publication:
16 Nov 2022
16 Nov 2022
Historique:
received:
14
08
2022
accepted:
25
10
2022
entrez:
17
11
2022
pubmed:
18
11
2022
medline:
22
11
2022
Statut:
epublish
Résumé
Pyrococcus furiosus is a hyperthermophilic archaeon with three effector CRISPR complexes (types I-A, I-B, and III-B) that each employ crRNAs derived from seven CRISPR arrays. Here, we investigate the CRISPR adaptation response to a newly discovered and self-transmissible plasmid, pT33.3. Transconjugant strains of Pyrococcus furiosus exhibited dramatically elevated levels of new spacer integration at CRISPR loci relative to the strain harboring a commonly employed, laboratory-constructed plasmid. High-throughput sequence analysis demonstrated that the vast majority of the newly acquired spacers were preferentially selected from DNA surrounding a particular region of the pT33.3 plasmid and exhibited a bi-directional pattern of strand bias that is a hallmark of primed adaptation by type I systems. We observed that one of the CRISPR arrays of our Pyrococcus furiosus laboratory strain encodes a spacer that closely matches the region of the conjugative plasmid that is targeted for adaptation. The hyper-adaptation phenotype was found to strictly depend both on the presence of this single matching spacer as well as the I-B effector complex, known to mediate primed adaptation. Our results indicate that Pyrococcus furiosus naturally encountered this conjugative plasmid or a related mobile genetic element in the past and responds to reinfection with robust primed adaptation.
Identifiants
pubmed: 36385310
doi: 10.1007/s00792-022-01281-0
pii: 10.1007/s00792-022-01281-0
pmc: PMC9838737
mid: NIHMS1859798
doi:
Substances chimiques
DNA
9007-49-2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
36Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM118140
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM118160
Pays : United States
Organisme : NIH HHS
ID : R35GM118140
Pays : United States
Organisme : NIH HHS
ID : R35GM118160
Pays : United States
Informations de copyright
© 2022. The Author(s), under exclusive licence to Springer Japan KK, part of Springer Nature.
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