Chromatin localization of nucleophosmin organizes ribosome biogenesis.
NPM1
chromatin
cryo-EM
fkbp
liquid-liquid phase separation
nascent 60S
nucleophosmin
ribosome biogenesis
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
01 12 2022
01 12 2022
Historique:
received:
26
10
2021
revised:
01
09
2022
accepted:
28
10
2022
pubmed:
25
11
2022
medline:
7
12
2022
entrez:
24
11
2022
Statut:
ppublish
Résumé
Ribosome biogenesis takes place in the nucleolus, a nuclear membrane-less organelle. Although well studied, it remains unknown how nascent ribosomal subunits separate from the central chromatin compartment and move to the outer granular component, where maturation occurs. We find that the Schizosaccharomyces pombe nucleophosmin-like protein Fkbp39 localizes to rDNA sites encoding the 60S subunit rRNA, and this localization contributes to its specific association with nascent 60S subunits. Fkbp39 dissociates from chromatin to bind nascent 60S subunits, causing the latter to partition away from chromatin and from nascent 40S subunits through liquid-liquid phase separation. In vivo, Fkbp39 binding directs the translocation of nascent 60S subunits toward the nucleophosmin-rich granular component. This process increases the efficiency of 60S subunit assembly, facilitating the incorporation of 60S RNA domain III. Thus, chromatin localization determines the specificity of nucleophosmin in sorting nascent ribosomal subunits and coordinates their movement into specialized assembly compartments within the nucleolus.
Identifiants
pubmed: 36423630
pii: S1097-2765(22)01061-9
doi: 10.1016/j.molcel.2022.10.033
pmc: PMC9949351
mid: NIHMS1849199
pii:
doi:
Substances chimiques
Chromatin
0
Nucleophosmin
117896-08-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
4443-4457.e9Subventions
Organisme : NIGMS NIH HHS
ID : R35 GM131891
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM135599
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM115634
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA021765
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM141694
Pays : United States
Informations de copyright
Copyright © 2022 The Author(s). Published by Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests R.W.K. reports personal fees from Dewpoint Therapeutics, GLG Consulting, and New Equilibrium Biosciences outside the submitted work.
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