Three Novel Antisense Overlapping Genes in E. coli O157:H7 EDL933.
EHEC O157:H7
competitive growth assays
overlapping genes
phenotype
prokaryotic genomes
protein
ribosome footprinting
Journal
Microbiology spectrum
ISSN: 2165-0497
Titre abrégé: Microbiol Spectr
Pays: United States
ID NLM: 101634614
Informations de publication
Date de publication:
14 02 2023
14 02 2023
Historique:
pubmed:
20
12
2022
medline:
17
2
2023
entrez:
19
12
2022
Statut:
ppublish
Résumé
The abundance of long overlapping genes in prokaryotic genomes is likely to be significantly underestimated. To date, only a few examples of such genes are fully established. Using RNA sequencing and ribosome profiling, we found expression of novel overlapping open reading frames in Escherichia coli O157:H7 EDL933 (EHEC). Indeed, the overlapping candidate genes are equipped with typical structural elements required for transcription and translation, i.e., promoters, transcription start sites, as well as terminators, all of which were experimentally verified. Translationally arrested mutants, unable to produce the overlapping encoded protein, were found to have a growth disadvantage when grown competitively against the wild type. Thus, the phenotypes found imply biological functionality of the genes at the level of proteins produced. The addition of 3 more examples of prokaryotic overlapping genes to the currently limited, yet constantly growing pool of such genes emphasizes the underestimated coding capacity of bacterial genomes.
Identifiants
pubmed: 36533921
doi: 10.1128/spectrum.02351-22
pmc: PMC9927249
doi:
Substances chimiques
Escherichia coli Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
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