Scanning aldoxime dehydratase sequence space and characterization of a new aldoxime dehydratase from Fusarium vanettenii.

Fusarium / genetics Hydro-Lyases / genetics Catalysis Oximes / metabolism
Aldoxime dehydratase Catalytic triad Fusarium vanettenii Homology modeling Nitrile synthesis Substrate docking

Journal

Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761

Informations de publication

Date de publication:
Mar 2023
Historique:
received: 04 09 2022
revised: 30 11 2022
accepted: 17 12 2022
pubmed: 8 1 2023
medline: 25 1 2023
entrez: 7 1 2023
Statut: ppublish

Résumé

The aim of this work was to map the sequence space of aldoxime dehydratases (Oxds) as enzymes with great potential for nitrile synthesis. Microbes contain an abundance of putative Oxds but fewer than ten Oxds were characterized in total and only two in fungi. In this work, we prepared and characterized a new Oxd (protein gb|EEU37245.1 named OxdFv) from Fusarium vanettenii 77-13-4. OxdFv is distant from the characterized Oxds with a maximum of 36% identity. Moreover, the canonical Oxd catalytic triad RSH is replaced by R141-E187-E303 in OxdFv. R141A and E187A mutants did not show significant activities, but mutant E303A showed a comparable activity as the wild-type enzyme. According to native mass spectrometry, OxdFv contained almost 1 mol of heme per 1 mol of protein, and was composed of approximately 88% monomer (41.8 kDa) and 12% dimer. A major advantage of this enzyme is its considerable activity under aerobic conditions (25.0 ± 4.3 U/mg for E,Z-phenylacetaldoxime at pH 9.0 and 55 °C). Addition of sodium dithionite (reducing agent) and Fe

Identifiants

DOI: 10.1016/j.enzmictec.2022.110187 PMID: 36610228
pubmed: 36610228
pii: S0141-0229(22)00206-X
doi: 10.1016/j.enzmictec.2022.110187
pii:
doi:

Substances chimiques

aldoxime dehydratase EC 4.2.1.-
Hydro-Lyases EC 4.2.1.-
Oximes 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

110187

Informations de copyright

Copyright © 2023 Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declarations of interest none.

Auteurs

Barbora Křístková (B)

Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ-142 20 Prague, Czech Republic; Faculty of Food and Biochemical Technology, University of Chemistry and Technology, Prague, Technická 5, CZ-166 28 Prague, Czech Republic.

Robert Rädisch (R)

Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ-142 20 Prague, Czech Republic; Department of Genetics and Microbiology, Faculty of Sciences, Charles University, Viničná 5, CZ-128 44 Prague, Czech Republic.

Natalia Kulik (N)

Laboratory of Structural Biology and Bioinformatics, Institute of Microbiology of the Czech Academy of Sciences, Zámek 136, CZ-373 33 Nové Hrady, Czech Republic.

Melissa Horvat (M)

Institute of Molecular Biotechnology, Faculty of Technical Chemistry, Chemical and Process Engineering, Biotechnology, Graz University of Technology, Petersgasse 14, A-8010 Graz, Austria.

Lenka Rucká (L)

Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ-142 20 Prague, Czech Republic.

Michal Grulich (M)

Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ-142 20 Prague, Czech Republic.

Florian Rudroff (F)

Institute of Applied Synthetic Chemistry, TU Wien, Getreidemarkt 9/OC-163, A-1060 Vienna, Austria.

Alan Kádek (A)

Laboratory of Structural Biology and Cell Signaling, BIOCEV - Institute of Microbiology, Czech Academy of Sciences, Průmyslová 595, CZ-252 50 Vestec, Czech Republic; Leibniz Institute of Virology (LIV), Martinistraße 52, D-20251 Hamburg, Germany; European XFEL GmbH, Holzkoppel 4, D-22869 Schenefeld, Germany.

Miroslav Pátek (M)

Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ-142 20 Prague, Czech Republic.

Margit Winkler (M)

Institute of Molecular Biotechnology, Faculty of Technical Chemistry, Chemical and Process Engineering, Biotechnology, Graz University of Technology, Petersgasse 14, A-8010 Graz, Austria; Austrian Center of Industrial Biotechnology GmbH, Krenngasse 37, A-8010 Graz, Austria.

Ludmila Martínková (L)

Institute of Microbiology of the Czech Academy of Sciences, Vídeňská 1083, CZ-142 20 Prague, Czech Republic. Electronic address: martinko@biomed.cas.cz.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Champignons › Deuteromycota › Fusarium › Scanning aldoxime dehydratase sequence space...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Lyases › Carbon-oxygen lyases › Hydro-lyases › Scanning aldoxime dehydratase sequence space...
questionsmedicales.fr › Phénomènes chimiques › Catalyse › Scanning aldoxime dehydratase sequence space...
questionsmedicales.fr › Composés chimiques organiques › Amines › Hydroxylamines › Oximes › Scanning aldoxime dehydratase sequence space...