The Medicago SymCEP7 hormone increases nodule number via shoots without compromising lateral root number.
Plant Root Nodulation
/ genetics
Plant Roots
/ metabolism
Medicago truncatula
/ metabolism
Rhizobium
/ physiology
Lotus
/ genetics
Peptides
/ metabolism
Trifolium
/ metabolism
Hormones
/ metabolism
Nitrogen
/ metabolism
Root Nodules, Plant
/ metabolism
Plant Proteins
/ genetics
Symbiosis
Gene Expression Regulation, Plant
Journal
Plant physiology
ISSN: 1532-2548
Titre abrégé: Plant Physiol
Pays: United States
ID NLM: 0401224
Informations de publication
Date de publication:
17 03 2023
17 03 2023
Historique:
received:
18
11
2022
accepted:
02
12
2022
pubmed:
19
1
2023
medline:
22
3
2023
entrez:
18
1
2023
Statut:
ppublish
Résumé
Legumes acquire soil nutrients through nitrogen-fixing root nodules and lateral roots. To balance the costs and benefits of nodulation, legumes negatively control root nodule number by autoregulatory and hormonal pathways. How legumes simultaneously coordinate root nodule and lateral root development to procure nutrients remains poorly understood. In Medicago (Medicago truncatula), a subset of mature C-TERMINALLY ENCODED PEPTIDE (CEP) hormones can systemically promote nodule number, but all CEP hormones tested to date negatively regulate lateral root number. Here we showed that Medicago CEP7 produces a mature peptide, SymCEP7, that promotes nodulation from the shoot without compromising lateral root number. Rhizobial inoculation induced CEP7 in the susceptible root nodulation zone in a Nod factor-dependent manner, and, in contrast to other CEP genes, its transcription level was elevated in the ethylene signaling mutant sickle. Using mass spectrometry, fluorescence microscopy and expression analysis, we demonstrated that SymCEP7 activity requires the COMPACT ROOT ARCHITECTURE 2 receptor and activates the shoot-to-root systemic effector, miR2111. Shoot-applied SymCEP7 rapidly promoted nodule number in the pM to nM range at concentrations up to five orders of magnitude lower than effects mediated by root-applied SymCEP7. Shoot-applied SymCEP7 also promoted nodule number in White Clover (Trifolium repens) and Lotus (Lotus japonicus), which suggests that this biological function may be evolutionarily conserved. We propose that SymCEP7 acts in the Medicago shoot to counter balance the autoregulation pathways induced rapidly by rhizobia to enable nodulation without compromising lateral root growth, thus promoting the acquisition of nutrients other than nitrogen to support their growth.
Identifiants
pubmed: 36653329
pii: 6991717
doi: 10.1093/plphys/kiad012
pmc: PMC10022606
doi:
Substances chimiques
Peptides
0
Hormones
0
Nitrogen
N762921K75
Plant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2012-2026Informations de copyright
© American Society of Plant Biologists 2023. All rights reserved. For permissions, please e-mail: journals.permissions@oup.com.
Déclaration de conflit d'intérêts
Conflict of interest statement. None declared.
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