Complementary DNA Significantly Enhancing Signal Response and Sensitivity of a Molecular Beacon Probe to Aflatoxin B1.
aflatoxin B1
aptamer
complementary DNA
enhanced signal
molecular beacon
Journal
Biosensors
ISSN: 2079-6374
Titre abrégé: Biosensors (Basel)
Pays: Switzerland
ID NLM: 101609191
Informations de publication
Date de publication:
28 Jan 2023
28 Jan 2023
Historique:
received:
22
12
2022
revised:
20
01
2023
accepted:
23
01
2023
entrez:
25
2
2023
pubmed:
26
2
2023
medline:
3
3
2023
Statut:
epublish
Résumé
This paper reported an improved molecular beacon method for the rapid detection of aflatoxin B1 (AFB1), a natural mycotoxin with severe carcinogenicity. With the assistance of a complementary DNA (cDNA) chain, the molecular beacon which consists of a DNA aptamer flanked by FAM and BHQ1 displayed a larger fluorescent response to AFB1, contributing to the sensitive detection of AFB1. Upon optimization of some key experimental factors, rapid detection of AFB1 ranging from 1 nM to 3 μM, within 20 min, was realized by using this method. A limit of detection (LoD) of 1 nM was obtained, which was lower than the LoD (8 nM) obtained without cDNA assistance. This aptamer-based molecular beacon detection method showed advantages in easy operation, rapid analysis and larger signal response. Good specificity and anti-interference ability were demonstrated. This method showed potential in real-sample analysis.
Identifiants
pubmed: 36831960
pii: bios13020195
doi: 10.3390/bios13020195
pmc: PMC9953557
pii:
doi:
Substances chimiques
DNA, Complementary
0
Aflatoxin B1
9N2N2Y55MH
Fluorescent Dyes
0
Molecular Probes
0
Aptamers, Nucleotide
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Shandong Provincial Natural Science Foundation of China
ID : ZR2021QB206
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