A Fluorophore-Labeled Lysine Dendrimer with an Oxo-Anion-Binding Motif for Tracking Gene Transfection.
DNA binding ligand
gene transfection
nonviral vectors
tracking
Journal
Chembiochem : a European journal of chemical biology
ISSN: 1439-7633
Titre abrégé: Chembiochem
Pays: Germany
ID NLM: 100937360
Informations de publication
Date de publication:
01 08 2023
01 08 2023
Historique:
revised:
17
04
2023
received:
12
04
2023
medline:
23
10
2023
pubmed:
19
4
2023
entrez:
18
4
2023
Statut:
ppublish
Résumé
A transfection vector based on a peptide dendrimer (1) has been developed and its abilities for DNA binding and transport have been investigated. By attaching a fluorophore to the vector system (1*), several steps in the transfection process could be monitored directly. As DLS and AFM studies showed, the labeled vector 1* condensed DNA into tightly packed aggregates able to enter eukaryotic cells. Co-localization experiments revealed that the ligand/plasmid complex is taken up by the endosomal pathway followed by an endosomal escape or lysosomal degradation. Afterwards, the plasmid DNA seems to enter the nucleus due to a breakdown of the nuclear envelope during mitosis, as only cells that have recently undergone mitosis showed H2B-GFP expression.
Identifiants
pubmed: 37071493
doi: 10.1002/cbic.202300296
doi:
Substances chimiques
Dendrimers
0
Lysine
K3Z4F929H6
DNA
9007-49-2
Anions
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202300296Informations de copyright
© 2023 The Authors. ChemBioChem published by Wiley-VCH GmbH.
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