Assembly and comparative genome analysis of a Patagonian Aureobasidium pullulans isolate reveals unexpected intraspecific variation.
Aureobasidium
RNA-seq
extremophiles
genetic speciation
genomics
oxidative stress
Journal
Yeast (Chichester, England)
ISSN: 1097-0061
Titre abrégé: Yeast
Pays: England
ID NLM: 8607637
Informations de publication
Date de publication:
May 2023
May 2023
Historique:
revised:
27
03
2023
received:
09
11
2022
accepted:
14
04
2023
medline:
12
6
2023
pubmed:
28
4
2023
entrez:
28
4
2023
Statut:
ppublish
Résumé
Aureobasidium pullulans is a yeast-like fungus with remarkable phenotypic plasticity widely studied for its importance for the pharmaceutical and food industries. So far, genomic studies with strains from all over the world suggest they constitute a genetically unstructured population, with no association by habitat. However, the mechanisms by which this genome supports so many phenotypic permutations are still poorly understood. Recent works have shown the importance of sequencing yeast genomes from extreme environments to increase the repertoire of phenotypic diversity of unconventional yeasts. In this study, we present the genomic draft of A. pullulans strain from a Patagonian yeast diversity hotspot, re-evaluate its taxonomic classification based on taxogenomic approaches, and annotate its genome with high-depth transcriptomic data. Our analysis suggests this isolate could be considered a novel variant at an early stage of the speciation process. The discovery of divergent strains in a genomically homogeneous group, such as A. pullulans, can be valuable in understanding the evolution of the species. The identification and characterization of new variants will not only allow finding unique traits of biotechnological importance, but also optimize the choice of strains whose phenotypes will be characterized, providing new elements to explore questions about plasticity and adaptation.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
197-213Subventions
Organisme : Comisión Nacional de Investigación Científica y Tecnológica
Organisme : National Science Foundation
Organisme : Great Lakes Bioenergy Research Center
Organisme : Fondo para la Investigación Científica y Tecnológica
Organisme : Universidad del Comahue
Organisme : National Institute of Food and Agriculture
Organisme : Consejo Nacional de Investigaciones Científicas y Técnicas
ID : PIP11220200102948CO
Organisme : DOE Great Lakes Bioenergy Research Center
ID : DE-SC0018409
Organisme : USDA National Institute of Food and Agriculture
ID : 1020204
Informations de copyright
© 2023 John Wiley & Sons Ltd.
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