Functional analysis of a random-sequence chromosome reveals a high level and the molecular nature of transcriptional noise in yeast cells.
biological function
biological noise
chromatin
polyadenylation
transcription
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
01 06 2023
01 06 2023
Historique:
received:
18
10
2022
revised:
18
02
2023
accepted:
10
04
2023
pmc-release:
01
06
2024
medline:
5
6
2023
pubmed:
4
5
2023
entrez:
3
5
2023
Statut:
ppublish
Résumé
We measure transcriptional noise in yeast by analyzing chromatin structure and transcription of an 18-kb region of DNA whose sequence was randomly generated. Nucleosomes fully occupy random-sequence DNA, but nucleosome-depleted regions (NDRs) are much less frequent, and there are fewer well-positioned nucleosomes and shorter nucleosome arrays. Steady-state levels of random-sequence RNAs are comparable to yeast mRNAs, although transcription and decay rates are higher. Transcriptional initiation from random-sequence DNA occurs at numerous sites, indicating very low intrinsic specificity of the RNA Pol II machinery. In contrast, poly(A) profiles of random-sequence RNAs are roughly comparable to those of yeast mRNAs, suggesting limited evolutionary restraints on poly(A) site choice. Random-sequence RNAs show higher cell-to-cell variability than yeast mRNAs, suggesting that functional elements limit variability. These observations indicate that transcriptional noise occurs at high levels in yeast, and they provide insight into how chromatin and transcription patterns arise from the evolved yeast genome.
Identifiants
pubmed: 37137302
pii: S1097-2765(23)00254-X
doi: 10.1016/j.molcel.2023.04.010
pmc: PMC10247422
mid: NIHMS1894461
pii:
doi:
Substances chimiques
Nucleosomes
0
Chromatin
0
RNA Polymerase II
EC 2.7.7.-
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
1786-1797.e5Subventions
Organisme : NIGMS NIH HHS
ID : F32 GM140555
Pays : United States
Organisme : NIGMS NIH HHS
ID : R01 GM030186
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM131801
Pays : United States
Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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