Decreased Ventral Tegmental Area CB1R Signaling Reduces Sign Tracking and Shifts Cue-Outcome Dynamics in Rat Nucleus Accumbens.


Journal

The Journal of neuroscience : the official journal of the Society for Neuroscience
ISSN: 1529-2401
Titre abrégé: J Neurosci
Pays: United States
ID NLM: 8102140

Informations de publication

Date de publication:
21 06 2023
Historique:
received: 21 07 2022
revised: 10 05 2023
accepted: 15 05 2023
pmc-release: 21 12 2023
medline: 23 6 2023
pubmed: 20 5 2023
entrez: 19 5 2023
Statut: ppublish

Résumé

Sign-tracking (ST) rats show enhanced cue sensitivity before drug experience that predicts greater discrete cue-induced drug seeking compared with goal-tracking or intermediate rats. Cue-evoked dopamine in the nucleus accumbens (NAc) is a neurobiological signature of sign-tracking behaviors. Here, we examine a critical regulator of the dopamine system, endocannabinoids, which bind the cannabinoid receptor-1 (CB1R) in the ventral tegmental area (VTA) to control cue-evoked striatal dopamine levels. We use cell type-specific optogenetics, intra-VTA pharmacology, and fiber photometry to test the hypothesis that VTA CB1R receptor signaling regulates NAc dopamine levels to control sign tracking. We trained male and female rats in a Pavlovian lever autoshaping (PLA) task to determine their tracking groups before testing the effect of VTA → NAc dopamine inhibition. We found that this circuit is critical for mediating the vigor of the ST response. Upstream of this circuit, intra-VTA infusions of rimonabant, a CB1R inverse agonist, during PLA decrease lever and increase food cup approach in sign-trackers. Using fiber photometry to measure fluorescent signals from a dopamine sensor, GRAB

Identifiants

pubmed: 37208179
pii: JNEUROSCI.1486-22.2023
doi: 10.1523/JNEUROSCI.1486-22.2023
pmc: PMC10286939
doi:

Substances chimiques

Dopamine VTD58H1Z2X
Endocannabinoids 0
Rimonabant RML78EN3XE
Polyesters 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Sous-ensembles de citation

IM

Pagination

4684-4696

Subventions

Organisme : NIDA NIH HHS
ID : F31 DA050367
Pays : United States
Organisme : NIDA NIH HHS
ID : R01 DA043533
Pays : United States

Informations de copyright

Copyright © 2023 the authors.

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Auteurs

Sam Z Bacharach (SZ)

Department of Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201.
Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland 21201.

David A Martin (DA)

Department of Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201.

Cassie A Stapf (CA)

Department of Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201.
Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland 21201.

Fangmiao Sun (F)

State Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
IDG/McGovern Institute for Brain Research at PKU, Peking University, Beijing 100871, People's Republic of China.
Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, People's Republic of China.

Yulong Li (Y)

State Key Laboratory of Membrane Biology, School of Life Sciences, Peking University, Beijing 100871, People's Republic of China.
IDG/McGovern Institute for Brain Research at PKU, Peking University, Beijing 100871, People's Republic of China.
Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, People's Republic of China.

Joseph F Cheer (JF)

Department of Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201.
Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland 21201.

Donna J Calu (DJ)

Department of Neurobiology, University of Maryland School of Medicine, Baltimore, Maryland 21201 dcalu@som.umaryland.edu.
Program in Neuroscience, University of Maryland School of Medicine, Baltimore, Maryland 21201.

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Classifications MeSH