Extracellular cytochrome nanowires appear to be ubiquitous in prokaryotes.
cryo-EM
cytochrome filaments
extracellular cytochrome nanowires
microbial nanowires
Journal
Cell
ISSN: 1097-4172
Titre abrégé: Cell
Pays: United States
ID NLM: 0413066
Informations de publication
Date de publication:
22 06 2023
22 06 2023
Historique:
received:
04
01
2023
revised:
04
03
2023
accepted:
10
05
2023
pmc-release:
22
06
2024
medline:
26
6
2023
pubmed:
9
6
2023
entrez:
8
6
2023
Statut:
ppublish
Résumé
Electrically conductive appendages from the anaerobic bacterium Geobacter sulfurreducens, recently identified as extracellular cytochrome nanowires (ECNs), have received wide attention due to numerous potential applications. However, whether other organisms employ similar ECNs for electron transfer remains unknown. Here, using cryoelectron microscopy, we describe the atomic structures of two ECNs from two major orders of hyperthermophilic archaea present in deep-sea hydrothermal vents and terrestrial hot springs. Homologs of Archaeoglobus veneficus ECN are widespread among mesophilic methane-oxidizing Methanoperedenaceae, alkane-degrading Syntrophoarchaeales archaea, and in the recently described megaplasmids called Borgs. The ECN protein subunits lack similarities in their folds; however, they share a common heme arrangement, suggesting an evolutionarily optimized heme packing for efficient electron transfer. The detection of ECNs in archaea suggests that filaments containing closely stacked hemes may be a common and widespread mechanism for long-range electron transfer in both prokaryotic domains of life.
Identifiants
pubmed: 37290436
pii: S0092-8674(23)00531-7
doi: 10.1016/j.cell.2023.05.012
pmc: PMC10330847
mid: NIHMS1903159
pii:
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Cytochromes
0
Heme
42VZT0U6YR
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
2853-2864.e8Subventions
Organisme : NIGMS NIH HHS
ID : K99 GM138756
Pays : United States
Organisme : NIGMS NIH HHS
ID : R00 GM138756
Pays : United States
Organisme : NIGMS NIH HHS
ID : R35 GM122510
Pays : United States
Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of interests The authors declare no competing interests.
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