Production of marine bacterial metalloprotease A69 and evaluation of its potential in preparing soybean peptides with angiotensin-converting enzyme-inhibitory activity.
Bacillus subtilis
angiotensin-converting enzyme-inhibitory activity
antihypertensive effects
marine bacterial metalloprotease
soybean peptides
Journal
Journal of the science of food and agriculture
ISSN: 1097-0010
Titre abrégé: J Sci Food Agric
Pays: England
ID NLM: 0376334
Informations de publication
Date de publication:
Nov 2023
Nov 2023
Historique:
revised:
07
06
2023
received:
19
03
2023
accepted:
20
06
2023
medline:
23
10
2023
pubmed:
20
6
2023
entrez:
20
6
2023
Statut:
ppublish
Résumé
Marine bacteria secrete a variety of proteases, which are a good source to explore proteases with application value. However, only a few marine bacterial proteases with a potential in bioactive peptides preparation have been reported. The metalloprotease A69 from the marine bacterium Anoxybacillus caldiproteolyticus 1A02591 was successfully expressed in the food safe bacterium Bacillus subtilis as a secreted enzyme. A technique to efficiently produce protease A69 in a 15-L bioreactor was established, with a production of 8988 U mL The marine bacterial metalloprotease A69 has a promising potential for preparing SPs with good nutritional and potential antihypertensive effects, laying a good foundation for its industrial production and application. © 2023 Society of Chemical Industry.
Sections du résumé
BACKGROUND
BACKGROUND
Marine bacteria secrete a variety of proteases, which are a good source to explore proteases with application value. However, only a few marine bacterial proteases with a potential in bioactive peptides preparation have been reported.
RESULTS
RESULTS
The metalloprotease A69 from the marine bacterium Anoxybacillus caldiproteolyticus 1A02591 was successfully expressed in the food safe bacterium Bacillus subtilis as a secreted enzyme. A technique to efficiently produce protease A69 in a 15-L bioreactor was established, with a production of 8988 U mL
CONCLUSION
CONCLUSIONS
The marine bacterial metalloprotease A69 has a promising potential for preparing SPs with good nutritional and potential antihypertensive effects, laying a good foundation for its industrial production and application. © 2023 Society of Chemical Industry.
Substances chimiques
Angiotensin-Converting Enzyme Inhibitors
0
Soybean Proteins
0
Peptides
0
Peptide Hydrolases
EC 3.4.-
Endopeptidases
EC 3.4.-
Metalloproteases
EC 3.4.-
Angiotensins
0
Peptidyl-Dipeptidase A
EC 3.4.15.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7153-7163Subventions
Organisme : National Natural Science Foundation of China
ID : U2006205
Organisme : National Natural Science Foundation of China
ID : 32200021
Informations de copyright
© 2023 Society of Chemical Industry.
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