Leaderless Bicistronic Design for Precise and Reliable Control of Gene Expression in

Corynebacterium glutamicum gene expression regulation improved orthogonality leaderless bicistronic design synthetic biology

Journal

ACS synthetic biology
ISSN: 2161-5063
Titre abrégé: ACS Synth Biol
Pays: United States
ID NLM: 101575075

Informations de publication

Date de publication:
21 07 2023
Historique:
medline: 27 7 2023
pubmed: 23 6 2023
entrez: 23 6 2023
Statut: ppublish

Résumé

In synthetic biology, the precise control of gene expression is challenging due to the limited orthogonality of expression elements. Here, to address this issue and improve the reusability of genetic elements, we developed a bicistronic expression cassette in

Identifiants

pubmed: 37350137
doi: 10.1021/acssynbio.3c00246
pmc: PMC10367133
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

2157-2167

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Auteurs

Xiuxia Liu (X)

National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214112, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China.

Manman Sun (M)

National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214112, China.
Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, U.K.

Alex Xiong Gao (AX)

Division of Life Science, The Hong Kong University of Science and Technology, Hong Kong, China.

Rodrigo Ledesma-Amaro (R)

Department of Bioengineering and Imperial College Centre for Synthetic Biology, Imperial College London, London SW7 2AZ, U.K.

Qiuwu Fang (Q)

Living Systems Institute, School of Biosciences, College of Life and Environmental Science, University of Exeter, Exeter EX4 4QD, U.K.

Yankun Yang (Y)

National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214112, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China.

Zhonghu Bai (Z)

National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing, Jiangnan University, Wuxi 214112, China.
Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.
Jiangsu Provincial Research Center for Bioactive Product Processing Technology, Jiangnan University, Wuxi 214122, China.

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