DNA selection by the master transcription factor PU.1.

Phylogeny Binding Sites Trans-Activators / metabolism DNA / metabolism
CD11B CP: Molecular biology CSF1R DNA methylation DNA specificity ETS transcription factors PU.1 SPI1 epigenetic regulation non-canonical binding protein-DNA interactions

Journal

Cell reports
ISSN: 2211-1247
Titre abrégé: Cell Rep
Pays: United States
ID NLM: 101573691

Informations de publication

Date de publication:
25 07 2023
Historique:
received: 13 12 2022
revised: 07 04 2023
accepted: 02 06 2023
medline: 31 7 2023
pubmed: 23 6 2023
entrez: 23 6 2023
Statut: ppublish

Résumé

The master transcriptional regulator PU.1/Spi-1 engages DNA sites with affinities spanning multiple orders of magnitude. To elucidate this remarkable plasticity, we have characterized 22 high-resolution co-crystallographic PU.1/DNA complexes across the addressable affinity range in myeloid gene transactivation. Over a purine-rich core (such as 5'-GGAA-3') flanked by variable sequences, affinity is negotiated by direct readout on the 5' flank via a critical glutamine (Q226) sidechain and by indirect readout on the 3' flank by sequence-dependent helical flexibility. Direct readout by Q226 dynamically specifies PU.1's characteristic preference for purines and explains the pathogenic mutation Q226E in Waldenström macroglobulinemia. The structures also reveal how disruption of Q226 mediates strand-specific inhibition by DNA methylation and the recognition of non-canonical sites, including the authentic binding sequence at the CD11b promoter. A re-synthesis of phylogenetic and structural data on the ETS family, considering the centrality of Q226 in PU.1, unifies the model of DNA selection by ETS proteins.

Identifiants

DOI: 10.1016/j.celrep.2023.112671 PMID: 37352101 PMC: PMC10479921
pubmed: 37352101
pii: S2211-1247(23)00682-4
doi: 10.1016/j.celrep.2023.112671
pmc: PMC10479921
mid: NIHMS1920472
pii:
doi:

Substances chimiques

proto-oncogene protein Spi-1 0
Trans-Activators 0
DNA 9007-49-2

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, U.S. Gov't, Non-P.H.S. Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

112671

Subventions

Organisme : NIGMS NIH HHS
ID : R01 GM111749
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL155178
Pays : United States
Organisme : NIGMS NIH HHS
ID : R21 GM137160
Pays : United States
Organisme : NCI NIH HHS
ID : R35 CA253127
Pays : United States

Informations de copyright

Copyright © 2023 The Author(s). Published by Elsevier Inc. All rights reserved.

Déclaration de conflit d'intérêts

Declaration of interests U.S. has received research funding from GlaxoSmithKline, Bayer Healthcare, Aileron Therapeutics, and Novartis; has received compensation for consultancy services and for serving on scientific advisory boards from GlaxoSmithKline, Bayer Healthcare, Celgene, Aileron Therapeutics, Stelexis Therapeutics, and Pieris Pharmaceuticals; and has equity ownership in and is serving on the board of directors of Stelexis Therapeutics.

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Auteurs

J Ross Terrell (JR)

Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.

Samuel J Taylor (SJ)

Departments of Cell Biology, Oncology, and Medicine, Ruth L. and David S. Gottesman Institute for Stem Cell Research and Regenerative Medicine, Blood Cancer Institute, and the Montefiore Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Amelia L Schneider (AL)

Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.

Yue Lu (Y)

Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.

Tyler N Vernon (TN)

Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.

Suela Xhani (S)

Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.

Ryan H Gumpper (RH)

Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA.

Ming Luo (M)

Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA; Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA.

W David Wilson (WD)

Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA; Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA.

Ulrich Steidl (U)

Departments of Cell Biology, Oncology, and Medicine, Ruth L. and David S. Gottesman Institute for Stem Cell Research and Regenerative Medicine, Blood Cancer Institute, and the Montefiore Einstein Cancer Center, Albert Einstein College of Medicine, Bronx, NY 10461, USA. Electronic address: ulrich.steidl@einsteinmed.edu.

Gregory M K Poon (GMK)

Department of Chemistry, Georgia State University, Atlanta, GA 30303, USA; Center for Diagnostics and Therapeutics, Georgia State University, Atlanta, GA 30303, USA. Electronic address: gpoon@gsu.edu.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Phylogenèse › DNA selection by the master transcription factor PU.1.
questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Sites de fixation › DNA selection by the master transcription factor PU.1.
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales régulatrices ou accessoires › Transactivateurs › DNA selection by the master transcription factor PU.1.
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ADN › DNA selection by the master transcription factor PU.1.