Identification and expression characteristics of NLP (NIN-like protein) gene family in pepper (Capsicum annuum L.).
Bioinformatics analysis
Gene expression
NLP transcription factor
Nitrogen stress
Pepper
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Aug 2023
Aug 2023
Historique:
received:
10
10
2022
accepted:
12
06
2023
medline:
31
7
2023
pubmed:
26
6
2023
entrez:
26
6
2023
Statut:
ppublish
Résumé
Pepper (Capsicum annum L.) is the main crop in the vegetable industry. The growth and development of peppers are regulated by nitrate, but there is limited research on the molecular mechanisms of nitrate absorption and assimilation in peppers. A plant specific transcription factor NLP plays an important role in nitrate signal transduction. In this study, a total of 7 NLP members were identified based on pepper genome data. Two nitrogen transport elements (GCN4) were found in the CaNLP5 promoter. In the phylogenetic tree, CaNLP members are divided into three branches, with pepper NLP and tomato NLP having the closest genetic relationship. The expression levels of CaNLP1, CaNLP3, and CaNLP4 are relatively high in the roots, stems, and leaves. The expression level of CaNLP7 gene is relatively high during the 5-7 days of pepper fruit color transformation. After various non-Biotic stress and hormone treatments, the expression of CaNLP1 was at a high level. The expression of CaNLP3 and CaNLP4 was down regulated in leaves, but up regulated in roots. Under conditions of nitrogen deficiency and sufficient nitrate, the expression patterns of NLP genes in pepper leaves and roots were determined. These results provide important insights into the multiple functions of CaNLPs in regulating nitrate absorption and transport.
Sections du résumé
BACKGROUND
BACKGROUND
Pepper (Capsicum annum L.) is the main crop in the vegetable industry. The growth and development of peppers are regulated by nitrate, but there is limited research on the molecular mechanisms of nitrate absorption and assimilation in peppers. A plant specific transcription factor NLP plays an important role in nitrate signal transduction.
METHODS AND RESULTS
RESULTS
In this study, a total of 7 NLP members were identified based on pepper genome data. Two nitrogen transport elements (GCN4) were found in the CaNLP5 promoter. In the phylogenetic tree, CaNLP members are divided into three branches, with pepper NLP and tomato NLP having the closest genetic relationship. The expression levels of CaNLP1, CaNLP3, and CaNLP4 are relatively high in the roots, stems, and leaves. The expression level of CaNLP7 gene is relatively high during the 5-7 days of pepper fruit color transformation. After various non-Biotic stress and hormone treatments, the expression of CaNLP1 was at a high level. The expression of CaNLP3 and CaNLP4 was down regulated in leaves, but up regulated in roots. Under conditions of nitrogen deficiency and sufficient nitrate, the expression patterns of NLP genes in pepper leaves and roots were determined.
CONCLUSION
CONCLUSIONS
These results provide important insights into the multiple functions of CaNLPs in regulating nitrate absorption and transport.
Identifiants
pubmed: 37358766
doi: 10.1007/s11033-023-08587-y
pii: 10.1007/s11033-023-08587-y
doi:
Substances chimiques
Nitrates
0
Transcription Factors
0
Nitrogen
N762921K75
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
6655-6668Subventions
Organisme : Genetic diversity analysis and core germplasm construction of pod pepper germplasm resources in southwest China
ID : 31760576
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
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