Molecular and morphological characterization of Xylaria karsticola (Ascomycota) isolated from the fruiting body of Macrolepiota procera (Basidiomycota) from Bulgaria.
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2023
2023
Historique:
received:
01
09
2022
accepted:
07
06
2023
medline:
3
7
2023
pubmed:
29
6
2023
entrez:
29
6
2023
Statut:
epublish
Résumé
The present study is the first to report Xylaria karsticola isolated from the basidiocarp of Macrolepiota procera (Basidiomycota), from Stara Planina Mountain, Bulgaria and second report for such species found in Europe. The fungal isolate was in vitro cultivated and the morphology was observed. It was primarily determined as a xylariaceous morphotype at the intragenus level, based on the evaluation of colony growth rate, color, and stromatic structure formation and was confirmed by unique conidiophores and conidia. The molecular identification of the isolate was performed by amplification of ITS1-5.8S-ITS2 region and the strain was identified as Xylaria karsticola with 97.57% of confidence. The obtained sequence was deposited in the GenBank database under the accession number MW996752 and in the National Bank of Industrial Microorganisms and Cell Cultures of Bulgaria under accession number NBIMCC 9097. The phylogenetic analysis of the isolate was also conducted by including 26 sequences obtained from different Xylaria isolates. Considering the phylogenetic data, X. karsticola NBIMCC 9097 was grouped along with other X. karsticola isolates, although the DNA sequence of the novel X. karsticola was rather distantly related to the other X. karsticola sequence data. The results were supported by the bootstrap analysis (100%) and indicated the different origin of the examined X. karsticola NBIMCC 9097.
Identifiants
pubmed: 37384635
doi: 10.1371/journal.pone.0287679
pii: PONE-D-22-24212
pmc: PMC10309620
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0287679Informations de copyright
Copyright: © 2023 Angelova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Déclaration de conflit d'intérêts
The authors have declared that no competing interests exist.
Références
Sci Rep. 2020 Mar 12;10(1):4599
pubmed: 32165688
Front Microbiol. 2021 Mar 30;12:629352
pubmed: 33859628
Org Lett. 2015 Sep 4;17(17):4284-7
pubmed: 26287562
Prog Mol Subcell Biol. 2012;53:229-41
pubmed: 22222835
FEMS Microbiol Lett. 2000 Jun 1;187(1):89-93
pubmed: 10828406
ISME J. 2021 May;15(5):1445-1457
pubmed: 33432137
J Nat Prod. 2011 Mar 25;74(3):395-401
pubmed: 21247198
Stud Mycol. 2014 Mar 15;77(1):1-143
pubmed: 24790283
Mycologia. 2009 Mar-Apr;101(2):256-68
pubmed: 19397200
J Mol Biol. 1990 Oct 5;215(3):403-10
pubmed: 2231712
J Antibiot (Tokyo). 2021 Jan;74(1):1-23
pubmed: 33097836
Curr Opin Microbiol. 2006 Aug;9(4):359-64
pubmed: 16777473
Plants (Basel). 2019 Oct 08;8(10):
pubmed: 31597306
Mol Biol Evol. 1987 Jul;4(4):406-25
pubmed: 3447015
J Fungi (Basel). 2021 Jul 22;7(8):
pubmed: 34436125
MycoKeys. 2022 Jan 12;86:47-63
pubmed: 35095304
Front Microbiol. 2016 Sep 29;7:1538
pubmed: 27746767
Mol Phylogenet Evol. 2010 Mar;54(3):957-69
pubmed: 20035889
PLoS One. 2013;8(3):e58268
pubmed: 23472167
Chem Biodivers. 2014 May;11(5):673-94
pubmed: 24827679
PLoS One. 2012;7(7):e40863
pubmed: 22808280
Mycologia. 2005 Jul-Aug;97(4):844-65
pubmed: 16457354
Mycol Res. 2007 Dec;111(Pt 12):1450-60
pubmed: 18023164
Mycologia. 2007 Nov-Dec;99(6):936-57
pubmed: 18333517
Am J Bot. 2012 May;99(5):898-914
pubmed: 22539507
Philos Trans R Soc Lond B Biol Sci. 2016 Oct 19;371(1706):
pubmed: 27619703
Mol Phylogenet Evol. 2016 May;98:210-32
pubmed: 26903035
J Microbiol Biotechnol. 2016 Jan;26(1):89-98
pubmed: 26428733