ASC oligomer favors caspase-1CARD domain recruitment after intracellular potassium efflux.
Journal
The Journal of cell biology
ISSN: 1540-8140
Titre abrégé: J Cell Biol
Pays: United States
ID NLM: 0375356
Informations de publication
Date de publication:
07 08 2023
07 08 2023
Historique:
received:
09
03
2020
revised:
21
02
2023
accepted:
28
04
2023
medline:
6
7
2023
pubmed:
4
7
2023
entrez:
4
7
2023
Statut:
ppublish
Résumé
Signaling through the inflammasome is important for the inflammatory response. Low concentrations of intracellular K+ are associated with the specific oligomerization and activation of the NLRP3 inflammasome, a type of inflammasome involved in sterile inflammation. After NLRP3 oligomerization, ASC protein binds and forms oligomeric filaments that culminate in large protein complexes named ASC specks. ASC specks are also initiated from different inflammasome scaffolds, such as AIM2, NLRC4, or Pyrin. ASC oligomers recruit caspase-1 and then induce its activation through interactions between their respective caspase activation and recruitment domains (CARD). So far, ASC oligomerization and caspase-1 activation are K+-independent processes. Here, we found that when there is low intracellular K+, ASC oligomers change their structure independently of NLRP3 and make the ASCCARD domain more accessible for the recruitment of the pro-caspase-1CARD domain. Therefore, conditions that decrease intracellular K+ not only drive NLRP3 responses but also enhance the recruitment of the pro-caspase-1 CARD domain into the ASC specks.
Identifiants
pubmed: 37402211
pii: 214211
doi: 10.1083/jcb.202003053
pmc: PMC10318405
pii:
doi:
Substances chimiques
CARD Signaling Adaptor Proteins
0
Caspase 1
EC 3.4.22.36
Inflammasomes
0
NLR Family, Pyrin Domain-Containing 3 Protein
0
Potassium
RWP5GA015D
Banques de données
Dryad
['10.5061/dryad.n8pk0p31b']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : European Research Council
ID : 899636
Pays : International
Informations de copyright
© 2023 Martín-Sánchez et al.
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