Genomic sequencing of fourteen bacillus thuringiensis isolates: insights into geographic variation and phylogenetic implications.
Bacillus thuringiensis
entomocidus
finitimus
israelensis
kurstaki
multilocus sequence type
pakistani
pesticidal proteins
toumanoffi
Journal
BMC research notes
ISSN: 1756-0500
Titre abrégé: BMC Res Notes
Pays: England
ID NLM: 101462768
Informations de publication
Date de publication:
04 Jul 2023
04 Jul 2023
Historique:
received:
27
09
2022
accepted:
20
06
2023
medline:
6
7
2023
pubmed:
5
7
2023
entrez:
4
7
2023
Statut:
epublish
Résumé
This work was performed in support of a separate study investigating the activity of pesticidal proteins produced by Bacillus thuringiensis against the Asian citrus psyllid, Diaphorina citri. The fourteen Bacillus isolates chosen were selected from a large, geographically diverse collection that was characterized only by biochemical phenotype and morphology of the parasporal crystal, hence, for each isolate it was desired to determine the specific pesticidal proteins produced, assign each to a Bacillus cereus multilocus sequence type (ST), and predict their placement within the classical Bt serotyping system. In addition, phylogenetic distances between the isolates and Bacillus thuringiensis serovar type strains were determined by calculating digital DNA-DNA hybridization (dDDH) values among the isolates. Based on the assembled sequence data, the isolates were found to be likely representatives of the Bt serovars kurstaki (ST 8), pakistani (ST 550), toumanoffi (ST 240), israelensis (ST 16), thuringiensis (ST 10), entomocidus (ST 239), and finitimus (ST 171). In cases where multiple isolates occurred within a predicted serovar, pesticidal protein profiles were found to be identical, despite the geographic diversity of the isolates. As expected, the dDDH values calculated for pairwise comparisons of the isolates and their apparent corresponding Bt serovar type strains were quite high (> 98%), however dDDH comparisons of the isolates with other serovar type strains were often surprisingly low (< 70%) and suggest unrecognized taxa within Bt and the Bacillus cereus sensu lato.
Identifiants
pubmed: 37403123
doi: 10.1186/s13104-023-06411-1
pii: 10.1186/s13104-023-06411-1
pmc: PMC10318680
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
134Subventions
Organisme : Emergency Citrus Diseases Research and Extension grants program, USDA National Institute of Food and Agriculture
ID : 2020-70029-33177
Organisme : Emergency Citrus Diseases Research and Extension grants program, USDA National Institute of Food and Agriculture
ID : 2020-70029-33177
Organisme : Emergency Citrus Diseases Research and Extension grants program, USDA National Institute of Food and Agriculture
ID : 2020-70029-33177
Organisme : Citrus Diseases Research and Extension grants program
ID : 2017-70016-26755
Organisme : Citrus Diseases Research and Extension grants program
ID : 2017-70016-26755
Informations de copyright
© 2023. This is a U.S. Government work and not under copyright protection in the US; foreign copyright protection may apply.
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