Discovery of two bifunctional/multifunctional cellulases by functional metagenomics.
Bifunctional/multifunctional cellulases
Endoglucanase
Functional metagenomics
Heterologous expression
β-glucosidase
Journal
Enzyme and microbial technology
ISSN: 1879-0909
Titre abrégé: Enzyme Microb Technol
Pays: United States
ID NLM: 8003761
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
25
04
2023
revised:
12
06
2023
accepted:
11
07
2023
medline:
27
7
2023
pubmed:
19
7
2023
entrez:
19
7
2023
Statut:
ppublish
Résumé
Cellulases are widely used in industry, and the usage in bioconversion of biofuels makes cellulases more valuable. In this study, two tandem genes that encoded cellulases ZF994-1 and ZF994-2, respectively, were identified on a cosmid from a soil metagenomic library. Phylogenetic analysis indicated that ZF994-1 and ZF994-2 belonged to glycoside hydrolase family 12 (GH12), and GH3, respectively. Based on the substrate specificity analysis, the recombinant ZF994-1 exhibited weak endoglucanase activity, moderate β-1,3-glucanase and β-1,4-mannanase activities, and strong β-glucosidase activity, while the recombinant ZF994-2 exhibited moderate endoglucanase activity and strong β-glucosidase activity. More than 45% β-glucosidase activity of the recombinant ZF994-1 retained in the buffer containing 3 M glucose, indicating the good tolerance against glucose. The recombinant ZF994-2 showed high activity in the presence of metal ions and organic reagents, exhibiting potential industrial applications.
Identifiants
pubmed: 37467538
pii: S0141-0229(23)00096-0
doi: 10.1016/j.enzmictec.2023.110288
pii:
doi:
Substances chimiques
Cellulases
EC 3.2.1.-
Cellulase
EC 3.2.1.4
Glucose
IY9XDZ35W2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
110288Informations de copyright
Copyright © 2023 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Conflict of interest There is no conflict of interest.