Identification of novel SNPs in Pun1 locus for pungency in Capsicum species.
Amino acid variation
Capsaicin
Capsicum
Pun 1
Pungency
SNP
Sequencing
Vitamin C
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Sep 2023
Sep 2023
Historique:
received:
13
11
2022
accepted:
18
07
2023
medline:
29
8
2023
pubmed:
29
7
2023
entrez:
29
7
2023
Statut:
ppublish
Résumé
Capsaicin and its analogues known as capsaicinoids are the principal sources of pungency in Capsicum spp. In this study, characterization of North-West Himalayan chilli germplasm and commercial landraces of different Indian states known for different pungency-color combinations was done based on capsaicin concentration. Moreover, molecular variation in pungency among high, medium and mild/not pungent Capsicum spp., especially those adapted to North-West Himalayas were elucidated. Forty-nine genotypes of chilli comprising breeding lines of Kashmiri origin, commercial landraces of Southern Indian origin and one of the world's hottest chilli Bhut Jolokia from Nagaland state of India were used as an experimental material. Wide variation in capsaicin content was observed among the genotypes, wherein, Bhut Jolokia (Capsicum chinense) expressed the highest capsaicin content (10,500.75 µg/g). Further, molecular analysis of PunI gene was done for discovering SNPs responsible for variations in pungency. In the non-pungent Nishat-1 (Capsicum annuum var. grossum), the 650 bp DNA fragment was not amplified due to 2.5 kb deletion spanning the putative promoter and first exon of AT3. The amplified DNA product for high and medium pungent was sequencing. Sequence alignment among revealed SNPs which were further observed responsible for variations in amino acid sequence and protein structure. The observed variation in protein structure might be responsible for high capsaicin production in one genotype as compared to the other and hence the protein conformation determines its interaction with the substrate.
Sections du résumé
BACKGROUND
BACKGROUND
Capsaicin and its analogues known as capsaicinoids are the principal sources of pungency in Capsicum spp. In this study, characterization of North-West Himalayan chilli germplasm and commercial landraces of different Indian states known for different pungency-color combinations was done based on capsaicin concentration. Moreover, molecular variation in pungency among high, medium and mild/not pungent Capsicum spp., especially those adapted to North-West Himalayas were elucidated.
METHODS AND RESULTS
RESULTS
Forty-nine genotypes of chilli comprising breeding lines of Kashmiri origin, commercial landraces of Southern Indian origin and one of the world's hottest chilli Bhut Jolokia from Nagaland state of India were used as an experimental material. Wide variation in capsaicin content was observed among the genotypes, wherein, Bhut Jolokia (Capsicum chinense) expressed the highest capsaicin content (10,500.75 µg/g). Further, molecular analysis of PunI gene was done for discovering SNPs responsible for variations in pungency. In the non-pungent Nishat-1 (Capsicum annuum var. grossum), the 650 bp DNA fragment was not amplified due to 2.5 kb deletion spanning the putative promoter and first exon of AT3. The amplified DNA product for high and medium pungent was sequencing. Sequence alignment among revealed SNPs which were further observed responsible for variations in amino acid sequence and protein structure.
CONCLUSION
CONCLUSIONS
The observed variation in protein structure might be responsible for high capsaicin production in one genotype as compared to the other and hence the protein conformation determines its interaction with the substrate.
Identifiants
pubmed: 37515708
doi: 10.1007/s11033-023-08691-z
pii: 10.1007/s11033-023-08691-z
doi:
Substances chimiques
Capsaicin
S07O44R1ZM
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
7571-7579Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
Références
Perry L, Dickau R, Zarrillo S, Holst I, Pearsall DM, Piperno DR, Berman MJ, Cooke RG, Rademaker K, Ranere AJ, RademakerK RAJ, Raymond JS, Sandweiss DH, Scaramelli F, Tarble K, Zeidler JA (2007) Starch fossils and the domestication and dispersal of chilli pepper (Capsicum spp. L) in the Americas. Science 315:986–988
doi: 10.1126/science.1136914
pubmed: 17303753
Salvador MH (2002) Genetic resources of chilli (Capsicum annuum L.) in Mexico. In: Proceedings of the 16th international pepper conference, Tampico, Tamaulipas, Mexico, pp 10–12
Purseglove JW (1977) Tropical crops. Dicotyledon—2. Longman. Green & Co. Publisher, London, pp 524–530
Jordth SE, Julius D (2002) Molecular basis for species specific sensitivity to ‘Hot’ chilli peppers. Cell 11:421–430
doi: 10.1016/S0092-8674(02)00637-2
Stewart C, Mazourek M, Stellari GM, Connell MO, Jahn M (2007) Genetic control of pungency in C. chinense via the PUN1 locus. J Exp Bot 58:979–991
doi: 10.1093/jxb/erl243
pubmed: 17339653
Suzuki T, Fujiwake H, Iwai K (1980) Intracellular localization of capsaicin and its analogues, capsaicinoid, in Capsicum fruit 1. Microscopic investigation of the structure of the placenta of Capsicum annuum var. annuum cv. Karayatsubusa. Plant Cell Physiol 21:839–853
doi: 10.1093/oxfordjournals.pcp.a076058
Prasad NBC, Gururaj HB, Kumar V, Giridhar P, Parimalan R, Sharma A, Ravi Shankar GA (2006) Influence of 8-methyl-Nonenoic acid on capsaicin biosynthesis invivo and invitro cell cultures of Capsicum spp. J Agric Federation Chem 54:1854–1859
doi: 10.1021/jf052085z
Harvell KP, Bosland PW (1997) The environment produces a significant effect on pungency of chiles (Capsicum annum L.). Hort Sci 32:1292
Iwai K, Suzuki T, Fujiwake H (1986) The formation and accumulation of pungent principle of hot pepper fruits, capsaicin and its analogues in Capsicum annuum var. annuum cv. Karayatsubusa at different growth stages after flowering. Agric Biol Chem 43:2493–2498
Bhattacharya A, Chattopadhyay A, Mazumdar D, Chakravarty A, Pal S (2010) Antioxidant constituents and enzymes activities in chilli peppers. Int J Veg Sci 16(3):201–211
doi: 10.1080/19315260903529709
Stewart C, Kang BC, Liu K, Mazourek M, Moore SL, Yoo EY, Kim BD, Paran I, Jahn MM (2005) The PUN1 gene for pungency in pepper encodes a putative acyltransferase. Plant J 42:675–688
doi: 10.1111/j.1365-313X.2005.02410.x
pubmed: 15918882
Stellari GM, Mazourek M, Jahn M (2010) Contrasting modes for loss of pungency between cultivated and wild species of Capsicum. Heredity 104(460):471
Reddy UK, Almeida A, Abburi VL, Alaparthi SB, Unselt D, Hankins G, Park M, Choi D, Nimmakayala P (2014) Identification of gene-specific polymorphisms and association with capsaicin pathway metabolites in Capsicum annuum L. collections. PLoS ONE 9(1):e86393
doi: 10.1371/journal.pone.0086393
pubmed: 24475113
pmcid: 3903536
Arce-Rodríguez ML, Ochoa-Alejo N (2015) Silencing AT3 gene reduces the expression of pAMT, BCAT, kas, and acl genes involved in capsaicinoid biosynthesis in chili pepper fruits. Biol Plant 59(3):477–484. https://doi.org/10.1007/s140535-015-0525-y
doi: 10.1007/s140535-015-0525-y
Ademoyegun OT, Fariyike TA, Aminu-Taiwo RB (2011) Effects of poultry dropping on the biologically active compounds in Capsicum annuum L. (var Nsukka yellow). Agric Biol J N Am 2(4):665–672
doi: 10.5251/abjna.2011.2.4.665.672
Doyle JJ, Doyle JL (1990) Isolation of plant DNA from fresh tissue. Focus 12:13–15
Bosland PW, Baral JB (2007) Bhut Jolokia-the World’s hottest known chile pepper is a putative naturally occurring interspecific hybrid. Hortic Sci 30:137–139
Popelka P, Jevinova P, Smejkal K, Roba P (2017) Determination of capsaicin content and pungency level of different fresh and dried chilli peppers. Folia Vet 2:11–16
doi: 10.1515/fv-2017-0012
Adluri PK, Borah AR, Nath PD (2017) Study of genetic diversity of Bhut Jolokia germplasm in north east India by SSR markers and morphology. Int J Pure Appl Biosci 5(4):1657–1665
doi: 10.18782/2320-7051.5625
Kirii E, Goto T, Yoshida Y, Yasuba K, Tanaka Y (2017) Non-pungency in a Japanese chilli pepper landrace (Capsicum annum) is caused by a novel loss-of-function PUN1 allele. Hortic J 86:61–69
doi: 10.2503/hortj.MI-148
Webber HJ (1911) Preliminary notes on pepper hybrids. J Hered 7:188–199
Blum E, Liu K, Mazourek M, Yoo EY, Jahn M, Paran I (2002) Molecular mapping of the C locus for presence of pungency in Capsicum. Genome 45:02–705
doi: 10.1139/g02-031
Zamora AG, Olvera C, Calleros AA, Hernandez JLG (2020) Genetic variability of Pun 1 gene (Capsaicin synthase) in pungent cultivars of Capsicum annuum of Northern Mexico. Trop Subtrop Agroecosyst 23:1–15
Yumnam JS, Tyagi W, Pandey A, Ngasepam TM (2012) Evaluation of genetic diversity of chilli landraces from North Eastern India based on morphology, SSR markers and the Pun 1 locus. Plant Mol Biol Report 30:1470–1479
doi: 10.1007/s11105-012-0466-y