Arabidopsis RNA polymerase II C-terminal domain phosphatase-like 1 targets mitogen-activated protein kinase cascades to suppress plant immunity.
Mitogen-Activated Protein Kinases
/ genetics
Arabidopsis
/ metabolism
RNA Polymerase II
/ metabolism
Mitogen-Activated Protein Kinase Kinases
/ metabolism
Arabidopsis Proteins
/ metabolism
Phosphoric Monoester Hydrolases
/ genetics
Gene Expression Regulation, Plant
Plant Immunity
/ genetics
Phosphoprotein Phosphatases
/ genetics
Transcription Factors
/ metabolism
RNA-Binding Proteins
/ metabolism
Arabidopsis
C-terminal domain (CTD) phosphatase-like 1 (CPL1)
microbe-associated molecular pattern (MAMP)
mitogen-activated protein kinase (MAPK)
pattern-triggered immunity (PTI)
Journal
Journal of integrative plant biology
ISSN: 1744-7909
Titre abrégé: J Integr Plant Biol
Pays: China (Republic : 1949- )
ID NLM: 101250502
Informations de publication
Date de publication:
Oct 2023
Oct 2023
Historique:
received:
21
03
2023
accepted:
31
07
2023
medline:
27
10
2023
pubmed:
3
8
2023
entrez:
3
8
2023
Statut:
ppublish
Résumé
Mitogen-activated protein kinase (MAPK) cascades play pivotal roles in plant defense against phytopathogens downstream of immune receptor complexes. The amplitude and duration of MAPK activation must be strictly controlled, but the underlying mechanism remains unclear. Here, we identified Arabidopsis CPL1 (C-terminal domain phosphatase-like 1) as a negative regulator of microbe-associated molecular pattern (MAMP)-triggered immunity via a forward-genetic screen. Disruption of CPL1 significantly enhanced plant resistance to Pseudomonas pathogens induced by the bacterial peptide flg22. Furthermore, flg22-induced MPK3/MPK4/MPK6 phosphorylation was dramatically elevated in cpl1 mutants but severely impaired in CPL1 overexpression lines, suggesting that CPL1 might interfere with flg22-induced MAPK activation. Indeed, CPL1 directly interacted with MPK3 and MPK6, as well as the upstream MKK4 and MKK5. A firefly luciferase-based complementation assay indicated that the interaction between MKK4/MKK5 and MPK3/MPK6 was significantly reduced in the presence of CPL1. These results suggest that CPL1 plays a novel regulatory role in suppressing MAMP-induced MAPK cascade activation and MAMP-triggered immunity to bacterial pathogens.
Substances chimiques
Mitogen-Activated Protein Kinases
EC 2.7.11.24
RNA Polymerase II
EC 2.7.7.-
Mitogen-Activated Protein Kinase Kinases
EC 2.7.12.2
Arabidopsis Proteins
0
Phosphoric Monoester Hydrolases
EC 3.1.3.2
CPL1 protein, Arabidopsis
EC 3.1.3.16
Phosphoprotein Phosphatases
EC 3.1.3.16
Transcription Factors
0
RNA-Binding Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
2380-2394Subventions
Organisme : National Natural Science Foundation of China
ID : 31671991
Informations de copyright
© 2023 Institute of Botany, Chinese Academy of Sciences.
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