Triacetic acid lactone production using 2-pyrone synthase expressing Yarrowia lipolytica via targeted gene deletion.

Yarrowia / genetics Gene Deletion Metabolic Engineering
Acetyl-CoA CRIPSR-cas9 Malonyl-CoA Metabolic engineering Polyketide Triacetic acid lactone Yarrowia lipolytica

Journal

Journal of bioscience and bioengineering
ISSN: 1347-4421
Titre abrégé: J Biosci Bioeng
Pays: Japan
ID NLM: 100888800

Informations de publication

Date de publication:
Oct 2023
Historique:
received: 14 03 2023
revised: 11 07 2023
accepted: 19 07 2023
medline: 15 9 2023
pubmed: 14 8 2023
entrez: 13 8 2023
Statut: ppublish

Résumé

An environmentally sustainable world can be realized by using microorganisms to produce value-added materials from renewable biomass. Triacetic acid lactone (TAL) is a high-value-added compound that is used as a precursor of various organic compounds such as food additives and pharmaceuticals. In this study, we used metabolic engineering to produce TAL from glucose using an oleaginous yeast Yarrowia lipolytica. We first introduced TAL-producing gene 2-pyrone synthase into Y. lipolytica, which enabled TAL production. Next, we increased TAL production by engineering acetyl-CoA and malonyl-CoA biosynthesis pathways by redirecting carbon flux to glycolysis. Finally, we optimized the carbon and nitrogen ratios in the medium, culminating in the production of 4078 mg/L TAL. The strategy presented in this study had the potential to improve the titer and yield of polyketide biosynthesis.

Identifiants

DOI: 10.1016/j.jbiosc.2023.07.006 PMID: 37574415
pubmed: 37574415
pii: S1389-1723(23)00233-5
doi: 10.1016/j.jbiosc.2023.07.006
pii:
doi:

Substances chimiques

triacetic acid lactone 675-10-5
2-pyrone 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

320-326

Informations de copyright

Copyright © 2023 The Society for Biotechnology, Japan. Published by Elsevier B.V. All rights reserved.

Auteurs

Yuta Matsuoka (Y)

Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.

Naofumi Fujie (N)

Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.

Mariko Nakano (M)

Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.

Ayumi Koshiba (A)

Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.

Akihiko Kondo (A)

Center for Sustainable Resource Science, RIKEN, 1-7-22 Suehiro-cho, Tsurumi-ku, Yokohama, Kanagawa 230-0045, Japan; Graduate School of Science, Technology and Innovation, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan.

Tsutomu Tanaka (T)

Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodai, Nada, Kobe 657-8501, Japan. Electronic address: tanaka@kitty.kobe-u.ac.jp.

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Classifications MeSH

questionsmedicales.fr › Eucaryotes › Champignons › Levures › Yarrowia › Triacetic acid lactone production using...
questionsmedicales.fr › Phénomènes génétiques › Mutagenèse › Délétion de séquence › Délétion de gène › Triacetic acid lactone production using...
questionsmedicales.fr › Technologie, industrie et agriculture › Ingénierie › Bioingénierie › Ingénierie cellulaire › Génie métabolique › Triacetic acid lactone production using...