Development of nanobodies against Staphylococcus enterotoxin B through yeast surface display.
Molecular docking
Nanobody
Staphylococcus enterotoxin B
Yeast surface display
Journal
International journal of biological macromolecules
ISSN: 1879-0003
Titre abrégé: Int J Biol Macromol
Pays: Netherlands
ID NLM: 7909578
Informations de publication
Date de publication:
31 Dec 2023
31 Dec 2023
Historique:
received:
27
07
2023
revised:
06
09
2023
accepted:
07
09
2023
medline:
23
11
2023
pubmed:
14
9
2023
entrez:
13
9
2023
Statut:
ppublish
Résumé
Staphylococcus enterotoxin B (SEB) is one of the primary virulence factors of Staphylococcus aureus but there is still a lack of targeted drugs. SEB activates immune cells via interacting with MHC-II on antigen-presenting cells, leading to the production of large amounts of pro-inflammatory cytokines. Blocking the interaction between SEB and MHC-II can avert the overactivation of immune cells. Nanobodies are the smallest functional antibodies that can bind stably to antigens. In this study, an ideal approach to obtain specific nanobodies without immunizing camelids was introduced. We constructed a library containing up to 5 × 10
Identifiants
pubmed: 37703983
pii: S0141-8130(23)03719-4
doi: 10.1016/j.ijbiomac.2023.126822
pii:
doi:
Substances chimiques
enterotoxin B, staphylococcal
39424-53-8
Single-Domain Antibodies
0
Enterotoxins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
126822Informations de copyright
Copyright © 2023. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.