The ester production capacity of Pichia kudriavzevii based on functional annotation of genes.
Baijiu
Esters
Gene annotation
HS-SPME-GC-MS
Pichia kudriavzevii
Volatile components
Journal
World journal of microbiology & biotechnology
ISSN: 1573-0972
Titre abrégé: World J Microbiol Biotechnol
Pays: Germany
ID NLM: 9012472
Informations de publication
Date de publication:
15 Sep 2023
15 Sep 2023
Historique:
received:
30
05
2023
accepted:
28
08
2023
medline:
18
9
2023
pubmed:
15
9
2023
entrez:
15
9
2023
Statut:
epublish
Résumé
Esters were identified as the primary volatile flavor compounds in Chinese Baijiu, exerting a significant influence on its quality and aroma. This study focused on the yeast strain Pichia kudriavzevii, renowned for its high capacity to produce esters. Whole genome sequences were annotated and analyzed using the GO, KEGG, KOG, CAZy, and Pfam databases to determine the genetic basis underly the enhanced ester production capacity. Results showed that P. kudriavzevii gene function was concentrated in biosynthetic capacity, metabolic capacity, amino acid translocation capacity, glycoside hydrolysis capacity and transfer capacity. Additionally, acyltransferase and kinase were predicted as active sites contributing to P. kudriavzevii high ester production. We further compared the volatile composition differences between P. kudriavzevii and Saccharomyces cerevisiae through Headspace solid-phase microextraction-gas Chromatography-mass spectrometry (HS-SPME-GC-MS), revealing P. kudriavzevii produced 3.5 times more esters than S. cerevisiae. Overall, our findings suggest that P. kudriavzevii had potential applications in the Baijiu brewing industry.
Identifiants
pubmed: 37713136
doi: 10.1007/s11274-023-03743-9
pii: 10.1007/s11274-023-03743-9
doi:
Substances chimiques
Amino Acids
0
Esters
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
307Subventions
Organisme : "13th Five-Year Plan" National Key R&D Program Project
ID : 2016YFD0400500
Organisme : "13th Five-Year Plan" National Key R&D Program Project
ID : 2016YFD0400500
Organisme : "13th Five-Year Plan" National Key R&D Program Project
ID : 2016YFD0400500
Organisme : "13th Five-Year Plan" National Key R&D Program Project
ID : 2016YFD0400500
Organisme : "13th Five-Year Plan" National Key R&D Program Project
ID : 2016YFD0400500
Organisme : Major special project of Hubei Provincial Science and Technology Department
ID : 2018ABA084
Organisme : Major special project of Hubei Provincial Science and Technology Department
ID : 2018ABA084
Organisme : Major special project of Hubei Provincial Science and Technology Department
ID : 2018ABA084
Organisme : Major special project of Hubei Provincial Science and Technology Department
ID : 2018ABA084
Organisme : Major special project of Hubei Provincial Science and Technology Department
ID : 2018ABA084
Informations de copyright
© 2023. The Author(s), under exclusive licence to Springer Nature B.V.
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