Genome sequence and characterization of Streptomyces phages Vanseggelen and Verabelle, representing two new species within the genus Camvirus.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
17 Nov 2023
17 Nov 2023
Historique:
received:
30
08
2023
accepted:
16
11
2023
medline:
27
11
2023
pubmed:
18
11
2023
entrez:
18
11
2023
Statut:
epublish
Résumé
Despite the rising interest in bacteriophages, little is known about their infection cycle and lifestyle in a multicellular host. Even in the model system Streptomyces, only a small number of phages have been sequenced and well characterized so far. Here, we report the complete characterization and genome sequences of Streptomyces phages Vanseggelen and Verabelle isolated using Streptomyces coelicolor as a host. A wide range of Streptomyces strains could be infected by both phages, but neither of the two phages was able to infect members of the closely related sister genus Kitasatospora. The phages Vanseggelen and Verabelle have a double-stranded DNA genome with lengths of 48,720 and 48,126 bp, respectively. Both phage genomes contain 72 putative genes, and the presence of an integrase encoding protein indicates a lysogenic lifestyle. Characterization of the phages revealed their stability over a wide range of temperatures (30-45 °C) and pH values (4-10). In conclusion, Streptomyces phage Vanseggelen and Streptomyces phage Verabelle are newly isolated phages that can be classified as new species in the genus Camvirus, within the subfamily Arquattrovirinae.
Identifiants
pubmed: 37978256
doi: 10.1038/s41598-023-47634-3
pii: 10.1038/s41598-023-47634-3
pmc: PMC10656467
doi:
Substances chimiques
DNA, Viral
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
20153Subventions
Organisme : NWO Vici
ID : VI.C.192.002
Informations de copyright
© 2023. The Author(s).
Références
Virus Res. 2019 May;265:34-42
pubmed: 30851301
Viruses. 2020 Sep 04;12(9):
pubmed: 32899836
Biochemistry. 2016 Aug 23;55(33):4614-25
pubmed: 27513288
J Virol Methods. 2004 Dec 15;122(2):147-52
pubmed: 15542138
Viruses. 2020 Sep 23;12(10):
pubmed: 32977693
Curr Protoc Bioinformatics. 2020 Jun;70(1):e102
pubmed: 32559359
J Bacteriol. 1959 Aug;78:186-92
pubmed: 13850163
Arch Virol. 2023 Jan 23;168(2):74
pubmed: 36683075
Viruses. 2020 Nov 06;12(11):
pubmed: 33172115
Nucleic Acids Res. 2018 Jan 4;46(D1):D708-D717
pubmed: 29040670
J Mol Biol. 2004 Jan 16;335(3):667-78
pubmed: 14687564
Bioinformatics. 2017 Mar 1;33(5):784-786
pubmed: 28365761
Biochem Soc Trans. 2013 Dec;41(6):1355-64
pubmed: 24256223
Sci Rep. 2022 Oct 22;12(1):17785
pubmed: 36273096
Arch Virol. 1992;124(3-4):201-9
pubmed: 1605737
Bioinformatics. 2017 Aug 01;33(15):2379-2380
pubmed: 28379287
Braz J Infect Dis. 2012 Sep-Oct;16(5):466-71
pubmed: 22975171
J Virol. 2018 Sep 26;92(20):
pubmed: 30089696
J Antibiot (Tokyo). 2017 May;70(5):506-513
pubmed: 28196972
Open Biol. 2022 Jun;12(6):210379
pubmed: 35673854
Nucleic Acids Res. 2013 Jan;41(Database issue):D36-42
pubmed: 23193287
Curr Opin Microbiol. 2023 Aug;74:102314
pubmed: 37030144
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2121-2129
pubmed: 15545445
Folia Microbiol (Praha). 2011 May;56(3):191-200
pubmed: 21625877
Int J Syst Bacteriol. 1992 Jan;42(1):156-60
pubmed: 1371059
J Gen Microbiol. 1973 May;76(1):163-76
pubmed: 4124710
BMC Bioinformatics. 2023 May 3;24(1):181
pubmed: 37131131
Can J Microbiol. 1981 Feb;27(2):254-7
pubmed: 7214241
Cell Genom. 2022 Nov 11;2(12):100213
pubmed: 36778052
Microbiology (Reading). 2014 Aug;160(Pt 8):1714-1725
pubmed: 24794971