Novel intragenic deletion within the FXN gene in a patient with typical phenotype of Friedreich ataxia: may be more prevalent than we think?

Biallelic expansion Deletion FXN Friedreich ataxia (FRDA) Parental testing

Journal

BMC medical genomics
ISSN: 1755-8794
Titre abrégé: BMC Med Genomics
Pays: England
ID NLM: 101319628

Informations de publication

Date de publication:
01 Dec 2023
Historique:
received: 05 12 2022
accepted: 18 11 2023
medline: 4 12 2023
pubmed: 2 12 2023
entrez: 2 12 2023
Statut: epublish

Résumé

Friedreich ataxia is the most common inherited ataxia in Europe and is mainly caused by biallelic pathogenic expansions of the GAA trinucleotide repeat in intron 1 of the FXN gene that lead to a decrease in frataxin protein levels. Rarely, affected individuals carry either a large intragenic deletion or whole-gene deletion of FXN on one allele and a full-penetrance expanded GAA repeat on the other allele. We report here a patient that presented the typical clinical features of FRDA and genetic analysis of FXN intron 1 led to the assumption that the patient carried the common biallelic expansion. Subsequently, parental sample testing led to the identification of a novel intragenic deletion involving the 5'UTR upstream region and exons 1 and 2 of the FXN gene by MLPA. With this case, we want to raise awareness about the potentially higher prevalence of intragenic deletions and underline the essential role of parental sample testing in providing accurate genetic counselling.

Sections du résumé

BACKGROUND BACKGROUND
Friedreich ataxia is the most common inherited ataxia in Europe and is mainly caused by biallelic pathogenic expansions of the GAA trinucleotide repeat in intron 1 of the FXN gene that lead to a decrease in frataxin protein levels. Rarely, affected individuals carry either a large intragenic deletion or whole-gene deletion of FXN on one allele and a full-penetrance expanded GAA repeat on the other allele.
CASE PRESENTATION METHODS
We report here a patient that presented the typical clinical features of FRDA and genetic analysis of FXN intron 1 led to the assumption that the patient carried the common biallelic expansion. Subsequently, parental sample testing led to the identification of a novel intragenic deletion involving the 5'UTR upstream region and exons 1 and 2 of the FXN gene by MLPA.
CONCLUSIONS CONCLUSIONS
With this case, we want to raise awareness about the potentially higher prevalence of intragenic deletions and underline the essential role of parental sample testing in providing accurate genetic counselling.

Identifiants

pubmed: 38041144
doi: 10.1186/s12920-023-01743-0
pii: 10.1186/s12920-023-01743-0
pmc: PMC10693098
doi:

Types de publication

Case Reports Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

312

Informations de copyright

© 2023. The Author(s).

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Auteurs

Cinthia Aguilera (C)

Genetics Laboratory, Laboratori Clínic Territorial Metropolitana Sud. Hospital Universitari de Bellvitge, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Spain. caguilerar@idibell.cat.

Anna Esteve-Garcia (A)

Clinical Genetics Unit, Laboratori Clínic Territorial Metropolitana Sud. Hospital Universitari de Bellvitge, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Spain.

Carlos Casasnovas (C)

Neuromuscular Unit, Neurology Department, Hospital Universitari de Bellvitge, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Spain.
Neurometabolic Diseases Group, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Spain.
Biomedical Research Network Centre in Rare Diseases (CIBERER), Madrid, Spain.

Valentina Vélez-Santamaria (V)

Neuromuscular Unit, Neurology Department, Hospital Universitari de Bellvitge, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Spain.

Laura Rausell (L)

Health in Code S.L, Valencia, Spain.

Pablo Gargallo (P)

Health in Code S.L, Valencia, Spain.

Javier Garcia-Planells (J)

Health in Code S.L, Valencia, Spain.

Pedro Alía (P)

Genetics Laboratory, Laboratori Clínic Territorial Metropolitana Sud. Hospital Universitari de Bellvitge, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Spain.

Núria Llecha (N)

Genetics Laboratory, Laboratori Clínic Territorial Metropolitana Sud. Hospital Universitari de Bellvitge, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Spain.
Clinical Genetics Unit, Laboratori Clínic Territorial Metropolitana Sud. Hospital Universitari de Bellvitge, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Spain.

Ariadna Padró-Miquel (A)

Genetics Laboratory, Laboratori Clínic Territorial Metropolitana Sud. Hospital Universitari de Bellvitge, Institut d'Investigació Biomèdica de Bellvitge (IDIBELL), L'Hospitalet de Llobregat, Spain. apadro@bellvitgehospital.cat.

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