Nonsense-mediated mRNA decay of metal-binding activator MAC1 is dependent on copper levels and 3'-UTR length in Saccharomyces cerevisiae.
Saccharomyces cerevisiae
/ genetics
Copper
/ metabolism
Saccharomyces cerevisiae Proteins
/ genetics
Nonsense Mediated mRNA Decay
3' Untranslated Regions
Gene Expression Regulation, Fungal
Transcription Factors
/ genetics
RNA, Messenger
/ genetics
Codon, Nonsense
/ genetics
Copper Transporter 1
Nuclear Proteins
Saccharomyces cerevisiae
3′-Untranslated regions (3′-UTRs)
Copper
Transcription factor
mRNA decay
mRNAs
Journal
Current genetics
ISSN: 1432-0983
Titre abrégé: Curr Genet
Pays: United States
ID NLM: 8004904
Informations de publication
Date de publication:
06 May 2024
06 May 2024
Historique:
received:
09
12
2023
accepted:
22
04
2024
revised:
12
04
2024
medline:
6
5
2024
pubmed:
6
5
2024
entrez:
6
5
2024
Statut:
epublish
Résumé
The nonsense-mediated mRNA decay (NMD) pathway was initially identified as a surveillance pathway that degrades mRNAs containing premature termination codons (PTCs). NMD is now also recognized as a post-transcriptional regulatory pathway that regulates the expression of natural mRNAs. Earlier studies demonstrated that regulation of functionally related natural mRNAs by NMD can be differential and condition-specific in Saccharomyces cerevisiae. Here, we investigated the regulation of MAC1 mRNAs by NMD in response to copper as well as the role the MAC1 3'-UTR plays in this regulation. MAC1 is a copper-sensing transcription factor that regulates the high-affinity copper uptake system. MAC1 expression is activated upon copper deprivation. We found that MAC1 mRNAs are regulated by NMD under complete minimal (CM) but escaped NMD under low and high copper conditions. Mac1 protein regulated gene, CTR1 is not regulated by NMD in conditions where MAC1 mRNAs are NMD sensitive. We also found that the MAC1 3'-UTR is the NMD targeting feature on the mRNAs, and that MAC1 mRNAs lacking 3'-UTRs were stabilized during copper deprivation. Our results demonstrate a mechanism of regulation for a metal-sensing transcription factor, at both the post-transcriptional and post-translational levels, where MAC1 mRNA levels are regulated by NMD and copper, while the activity of Mac1p is controlled by copper levels.
Identifiants
pubmed: 38709348
doi: 10.1007/s00294-024-01291-9
pii: 10.1007/s00294-024-01291-9
doi:
Substances chimiques
Copper
789U1901C5
Saccharomyces cerevisiae Proteins
0
3' Untranslated Regions
0
Transcription Factors
0
RNA, Messenger
0
MAC1 protein, S cerevisiae
0
Codon, Nonsense
0
CTR1 protein, S cerevisiae
0
Copper Transporter 1
0
Nuclear Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5Subventions
Organisme : NIGMS NIH HHS
ID : R15GM117524.
Pays : United States
Organisme : NIGMS NIH HHS
ID : R15GM117524.
Pays : United States
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
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