CmERF1 acts as a positive regulator of fruits and leaves growth in melon (Cucumis melo L.).
Cucumis melo
/ genetics
Plant Leaves
/ genetics
Gene Expression Regulation, Plant
Plant Proteins
/ genetics
Plant Growth Regulators
/ metabolism
Fruit
/ genetics
Plants, Genetically Modified
Cyclopentanes
/ pharmacology
Promoter Regions, Genetic
Oxylipins
/ pharmacology
Abscisic Acid
/ metabolism
Arabidopsis
/ genetics
Indoleacetic Acids
/ metabolism
Acetates
/ pharmacology
Salicylic Acid
/ metabolism
CmCDC48
CmERF1
Fruits and leaves development
Melon
Journal
Plant molecular biology
ISSN: 1573-5028
Titre abrégé: Plant Mol Biol
Pays: Netherlands
ID NLM: 9106343
Informations de publication
Date de publication:
06 Jun 2024
06 Jun 2024
Historique:
received:
19
01
2024
accepted:
14
05
2024
medline:
6
6
2024
pubmed:
6
6
2024
entrez:
6
6
2024
Statut:
epublish
Résumé
Melon (Cucumis melo L.) is an important horticultural and economic crop. ETHYLENE RESPONSE FACTOR1 (ERF1) plays an important role in regulating plant development, and the resistance to multiple biotic and abiotic stresses. In this study, developmental biology, molecular biology and biochemical assays were performed to explore the biological function of CmERF1 in melon. Abundant transcripts of CmERF1 were found in ovary at green-yellow bud (GYB) and rapid enlargement (ORE) stages. In CmERF1 promoter, the cis-regulatory elements for indoleacetic acid (IAA), methyl jasmonate (MeJA), salicylic acid (SA), abscisic acid (ABA), gibberellic acid (GA), light and low temperature responses were found. CmERF1 could be significantly induced by ethylene, IAA, MeJA, SA, ABA, and respond to continuous light and low temperature stresses in melon. Ectopic expression of CmERF1 increased the length of siliqua and carpopodium, and expanded the size of leaves in Arabidopsis. Knockdown of CmERF1 led to smaller ovary at anthesis, mature fruit and leaves in melon. In CmERF1-RNAi #2 plants, 75 genes were differently expressed compared with control, and the promoter regions of 28 differential expression genes (DEGs) contained the GCC-box (AGCCGCC) or DRE (A/GCCGAC) cis-acting elements of CmERF1. A homolog of cell division cycle protein 48 (CmCDC48) was proved to be the direct target of CmERF1 by the yeast one-hybrid assay and dual-luciferase (LUC) reporter (DLR) system. These results indicated that CmERF1 was able to promote the growth of fruits and leaves, and involved in multiple hormones and environmental signaling pathways in melon.
Identifiants
pubmed: 38842600
doi: 10.1007/s11103-024-01468-3
pii: 10.1007/s11103-024-01468-3
doi:
Substances chimiques
Plant Proteins
0
Plant Growth Regulators
0
Cyclopentanes
0
Oxylipins
0
methyl jasmonate
900N171A0F
Abscisic Acid
72S9A8J5GW
Indoleacetic Acids
0
Acetates
0
indoleacetic acid
6U1S09C61L
Salicylic Acid
O414PZ4LPZ
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
70Subventions
Organisme : National Natural Science Foundation of China
ID : 32002063
Organisme : Natural Science Foundation of Hebei Province
ID : C2021204033
Organisme : Science and Technology Project of Hebei Education Department
ID : BJK2023111
Organisme : Earmarked Fund for Hebei Vegetables Innovation Team of Modern Agro-industry Technology Research System
ID : HBCT2023100208
Organisme : Special Research Foundation of Hebei Agricultural University
ID : YJ201837
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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