Immunodetection of tubulin and centromeric histone H3 (CENH3) proteins in Glycine species.
Glycine
Centromeric histone H3 (CENH3)
Immunofluorescence
Soybean
Tubulin
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
13 Jul 2024
13 Jul 2024
Historique:
received:
30
04
2024
accepted:
16
06
2024
medline:
14
7
2024
pubmed:
14
7
2024
entrez:
13
7
2024
Statut:
epublish
Résumé
The centromeres appear as primary constrictions on monocentric metaphase chromosomes; where sister chromatids are held together and assemble the proteinaceous kitechore complex at which microtubule proteins attach during nuclear divisions for pulling sister chromatids to opposite cell poles. The movement of chromosomes is usually governed by structural proteins that are either species-specific or highly conserved, such as the centromere-specific histone H3 (CENH3) and tubulin proteins, respectively. We aimed to detect these proteins across eight different Glycine species by an immunofluorescence assay using specific antibodies. Furthermore, with the α-tubulin antibody we traced the dynamics of microtubules during the mitotic cell cycle in Glycine max. With two-color immunofluorescence staining, we showed that both proteins interact during nuclear division. Finally, we proved that in different diploid and tetraploid Glycine species CENH3 can be detected in functional centromeres with spatial proximity of microtubule proteins.
Sections du résumé
BACKGROUND
BACKGROUND
The centromeres appear as primary constrictions on monocentric metaphase chromosomes; where sister chromatids are held together and assemble the proteinaceous kitechore complex at which microtubule proteins attach during nuclear divisions for pulling sister chromatids to opposite cell poles. The movement of chromosomes is usually governed by structural proteins that are either species-specific or highly conserved, such as the centromere-specific histone H3 (CENH3) and tubulin proteins, respectively.
METHODS AND RESULTS
RESULTS
We aimed to detect these proteins across eight different Glycine species by an immunofluorescence assay using specific antibodies. Furthermore, with the α-tubulin antibody we traced the dynamics of microtubules during the mitotic cell cycle in Glycine max. With two-color immunofluorescence staining, we showed that both proteins interact during nuclear division.
CONCLUSIONS
CONCLUSIONS
Finally, we proved that in different diploid and tetraploid Glycine species CENH3 can be detected in functional centromeres with spatial proximity of microtubule proteins.
Identifiants
pubmed: 39001981
doi: 10.1007/s11033-024-09730-z
pii: 10.1007/s11033-024-09730-z
doi:
Substances chimiques
Histones
0
Tubulin
0
Glycine
TE7660XO1C
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
792Subventions
Organisme : Turkish Council of Higher Education, Türkiye
ID : Graduate School 100/2000 YÖK Program
Organisme : Ayhan Şahenk Foundation, Türkiye
ID : Graduate School Scholarship
Organisme : Türkiye Bilimsel ve Teknolojik Araştırma Kurumu
ID : 121O014
Organisme : Niğde Ömer Halisdemir University, Türkiye
ID : TGT2021/14-LÜTEP
Informations de copyright
© 2024. The Author(s), under exclusive licence to Springer Nature B.V.
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