Transcriptome, hormonal, and secondary metabolite changes in leaves of DEFENSE NO DEATH 1 (DND1) silenced potato plants.
Solanum tuberosum
/ genetics
Plant Leaves
/ metabolism
Gene Expression Regulation, Plant
Transcriptome
Plant Proteins
/ genetics
Cyclopentanes
/ metabolism
Plant Diseases
/ genetics
Gene Silencing
Disease Resistance
/ genetics
Plant Growth Regulators
/ metabolism
Oxylipins
/ metabolism
Gene Expression Profiling
Salicylic Acid
/ metabolism
Secondary Metabolism
/ genetics
Solanum tuberosum
Differentially expressed genes
Disease resistance genes
Metabolite analysis
Salicylic acid
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
04 Sep 2024
04 Sep 2024
Historique:
received:
03
05
2024
accepted:
27
08
2024
medline:
5
9
2024
pubmed:
5
9
2024
entrez:
4
9
2024
Statut:
epublish
Résumé
DEFENSE NO DEATH 1 (DND1) is a cyclic nucleotide-gated ion channel protein. Earlier, it was shown that the silencing of DND1 in the potato (Solanum tuberosum L.) leads to resistance to late blight, powdery mildew, and gray mold diseases. At the same time, however, it can reduce plant growth and cause leaf necrosis. To obtain knowledge of the molecular events behind the pleiotropic effect of DND1 downregulation in the potato, metabolite and transcriptome analyses were performed on three DND1 silenced lines of the cultivar 'Désirée.' A massive increase in the salicylic acid content of leaves was detected. Concentrations of jasmonic acid and chlorogenic acid and their derivatives were also elevated. Expression of 1866 genes was altered in the same way in all three DND1 silenced lines, including those related to the synthesis of secondary metabolites. The activation of several alleles of leaf rust, late blight, and other disease resistance genes, as well as the induction of pathogenesis-related genes, was detected. WRKY and NAC transcription factor families were upregulated, whereas bHLHs were downregulated, indicating their central role in transcriptome changes. These results suggest that the maintenance of the constitutive defense state leads to the reduced growth of DND1 silenced potato plants.
Identifiants
pubmed: 39232097
doi: 10.1038/s41598-024-71380-9
pii: 10.1038/s41598-024-71380-9
doi:
Substances chimiques
Plant Proteins
0
Cyclopentanes
0
Plant Growth Regulators
0
jasmonic acid
6RI5N05OWW
Oxylipins
0
Salicylic Acid
O414PZ4LPZ
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
20601Subventions
Organisme : National Research, Development and Innovation Office
ID : K_132967
Informations de copyright
© 2024. The Author(s).
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