Mycobacterial HelD connects RNA polymerase recycling with transcription initiation.
Mycobacterium smegmatis
/ metabolism
DNA-Directed RNA Polymerases
/ metabolism
Bacterial Proteins
/ metabolism
Rifampin
/ pharmacology
Sigma Factor
/ metabolism
Promoter Regions, Genetic
Transcription Initiation, Genetic
Transcription Factors
/ metabolism
Adenosine Triphosphate
/ metabolism
Transcription, Genetic
Gene Expression Regulation, Bacterial
Protein Binding
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
09 Oct 2024
09 Oct 2024
Historique:
received:
21
12
2023
accepted:
23
09
2024
medline:
10
10
2024
pubmed:
10
10
2024
entrez:
9
10
2024
Statut:
epublish
Résumé
Mycobacterial HelD is a transcription factor that recycles stalled RNAP by dissociating it from nucleic acids and, if present, from the antibiotic rifampicin. The rescued RNAP, however, must disengage from HelD to participate in subsequent rounds of transcription. The mechanism of release is unknown. We show that HelD from Mycobacterium smegmatis forms a complex with RNAP associated with the primary sigma factor σ
Identifiants
pubmed: 39384756
doi: 10.1038/s41467-024-52891-5
pii: 10.1038/s41467-024-52891-5
doi:
Substances chimiques
DNA-Directed RNA Polymerases
EC 2.7.7.6
Bacterial Proteins
0
Rifampin
VJT6J7R4TR
Sigma Factor
0
Transcription Factors
0
Adenosine Triphosphate
8L70Q75FXE
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
8740Informations de copyright
© 2024. The Author(s).
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