Molecular identification and studies on genetic diversity and structure-related GC heterogeneity of Spatholobus Suberectus based on ITS2.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
09 10 2024
09 10 2024
Historique:
received:
07
07
2024
accepted:
08
10
2024
medline:
10
10
2024
pubmed:
10
10
2024
entrez:
9
10
2024
Statut:
epublish
Résumé
To determine the role of internal transcribed spacer 2 (ITS2) in the identification of Spatholobus suberectus and explore the genetic diversity of S. suberectus. A total of 292 ITS2s from S. suberectus and 17 other plant species were analysed. S. suberectus was clustered separately in the phylogenetic tree. The genetic distance between species was greater than that within S. suberectus. Synonymous substitution rate (Ks) analysis revealed that ITS2 diverged the most recently within S. suberectus (Ks = 0.0022). These findings suggested that ITS2 is suitable for the identification of S. suberectus. The ITS2s were divided into 8 haplotypes and 4 evolutionary branches on the basis of secondary structure, indicating that there was variation within S. suberectus. Evolutionary analysis revealed that the GC content of paired regions (pGC) was greater than that of unpaired regions (upGC), and the pGC showed a decreasing trend, whereas the upGC remained unchanged. Single-base mutation was the main cause of base pair substitution. In both the initial state and the equilibrium state, the substitution rate of GC was higher than that of AU. The increase in the GC content was partly attributed to GC-biased gene conversion (gBGC). High GC content reflected the high recombination and mutation rates of ITS2, which is the basis for species identification and genetic diversity. We characterized the sequence and structural characteristics of S. suberectus ITS2 in detail, providing a reference and basis for the identification of S. suberectus and its products, as well as the protection and utilization of wild resources.
Identifiants
pubmed: 39384849
doi: 10.1038/s41598-024-75763-w
pii: 10.1038/s41598-024-75763-w
doi:
Substances chimiques
DNA, Ribosomal Spacer
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
23523Subventions
Organisme : Survey and Collection of Germplasm Resources of Woody & Herbaceous Plants in Guangxi, China
ID : GXFS-2021-34
Organisme : Self-funded scientific research project of Guangxi Zhuang Autonomous Region Administration of Traditional Chinese Medicine "Investigation and quality evaluation of germplasm resources of Spatholobus suberectus in Guangxi production areas
ID : GXZYA20220013
Organisme : Guangxi Traditional Chinese Medicine Appropriate Technology Development and Promotion Project "Optimal solutions for suitable niches for six key protected wild medicinal plants in Guangxi based on photosynthetic characteristics
ID : GZSY22-06
Organisme : Guangxi High-Level Key Disciplines Construction Pilot Project in Traditional Chinese Medicine-Authentication of Chinese Medicinal Materials
ID : No. 27
Informations de copyright
© 2024. The Author(s).
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