Microbial community acclimation during anaerobic digestion of high-oil food waste.

Anaerobiosis Methane / metabolism Sewage / microbiology Microbiota RNA, Ribosomal, 16S / genetics Bacteria / classification Fatty Acids, Volatile / metabolism High-Throughput Nucleotide Sequencing Acclimatization Food Archaea / metabolism Oils / metabolism Biodegradation, Environmental Food Loss and Waste

Journal

Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288

Informations de publication

Date de publication:
25 Oct 2024
Historique:
received: 09 07 2024
accepted: 21 10 2024
medline: 26 10 2024
pubmed: 26 10 2024
entrez: 25 10 2024
Statut: epublish

Résumé

Anaerobic digestion is one of the most promising options for the disposal of biodegradable food waste. However, the relatively high content of oil in food waste inhibits the conversion efficiency of anaerobic digestion because of the accumulation of long-chain fatty acids (LCFAs). In this study, activated anaerobic sludge was acclimated to accommodate high-oil conditions. The methane yield of high-oil food waste digested by the acclimated sludge increased by 24.9% compared to that digested by the raw sludge. To determine the internal changes in the acclimated sludge, the shifts in the microbial communities during the acclimation period were investigated via high-throughput sequencing (HTS) based on the 16 S rRNA gene. The results indicated that Bacteroidetes, Firmicutes, Chloroflexi and Proteobacteria were the dominant bacteria at the phylum level. The acclimation time allows some functional bacterial taxa to proliferate, such as Clostridium and Longilinea, which are able to degrade LCFAs and turn them into small organic molecules that also have nutrient value for other bacteria. Among the archaeal communities, the hydrogenotrophic methanogen Methanobacterium nearly supplanted the acetotrophic methanogen Methanosaeta. The time profiles of volatile fatty acids (VFAs) and pH during this period provided additional evidence for the success of the acclimation. This study demonstrated the effectiveness of acclimation and the dynamic of microbial communities, which further contributed to the management and resource utilization of high-oil food waste.

Identifiants

DOI: 10.1038/s41598-024-77136-9 PMID: 39455737
pubmed: 39455737
doi: 10.1038/s41598-024-77136-9
pii: 10.1038/s41598-024-77136-9
doi:

Substances chimiques

Methane OP0UW79H66
Sewage 0
RNA, Ribosomal, 16S 0
Fatty Acids, Volatile 0
Oils 0
Food Loss and Waste 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Pagination

25364

Informations de copyright

© 2024. The Author(s).

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Auteurs

Yangqing Hu (Y)

Fair Friend Institute of Intelligent Manufacturing, Hangzhou Vocational and Technical College, Hangzhou, 310018, China. huyangqing@zju.edu.cn.
Hangzhou Huaxin Mechanical and Electrical Engineering Co., Ltd, Hangzhou, 310030, China. huyangqing@zju.edu.cn.
College of Electronic Information and Optical Engineering, Nankai University, Tianjin, 300350, China. huyangqing@zju.edu.cn.

Zhaozhi Zhou (Z)

Zhejiang Development & Planning Institute, Hangzhou, 310012, China.

Ce Shen (C)

Hangzhou Huaxin Mechanical and Electrical Engineering Co., Ltd, Hangzhou, 310030, China.

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Classifications MeSH

questionsmedicales.fr › Métabolisme › Anaérobiose › Microbial community acclimation during...
questionsmedicales.fr › Composés chimiques organiques › Hydrocarbures › Hydrocarbures acycliques › Alcanes › Méthane › Microbial community acclimation during...
questionsmedicales.fr › Mélanges complexes › Eaux d'égout › Microbial community acclimation during...
questionsmedicales.fr › Environnement et santé publique › Environnement › Écosystème › Biodiversité › Biote › Microbiote › Microbial community acclimation during...
questionsmedicales.fr › Acides nucléiques, nucléotides et nucléosides › Acides nucléiques › ARN › ARN ribosomique › ARN ribosomique 16S › Microbial community acclimation during...
questionsmedicales.fr › Bactéries › Microbial community acclimation during...
questionsmedicales.fr › Lipides › Acides gras › Acides gras volatils › Microbial community acclimation during...
questionsmedicales.fr › Techniques d'investigation › Techniques génétiques › Analyse de séquence › Séquençage nucléotidique à haut débit › Microbial community acclimation during...
questionsmedicales.fr › Phénomènes biologiques › Adaptation biologique › Adaptation physiologique › Acclimatation › Microbial community acclimation during...
questionsmedicales.fr › Aliments et boissons › Aliments › Microbial community acclimation during...
questionsmedicales.fr › Archéobactéries › Microbial community acclimation during...
questionsmedicales.fr › Lipides › Huiles › Microbial community acclimation during...
questionsmedicales.fr › Environnement et santé publique › Santé publique › Pollution de l'environnement › Assainissement et restauration de l'environnement › Dépollution biologique de l'environnement › Microbial community acclimation during...