Institute of Rice Industry Technology Research, Key Laboratory of Functional Agriculture of Guizhou Provincial, Department of Education, Key Laboratory of Molecular Breeding for Grain and oil Crops in Guizhou Province, College of Agricultural Sciences, Guizhou University, Guiyang, 550025, China.
Publications dans "Systèmes de transport d'acides aminés neutres" :
Institute of Rice Industry Technology Research, Key Laboratory of Functional Agriculture of Guizhou Provincial, Department of Education, Key Laboratory of Molecular Breeding for Grain and oil Crops in Guizhou Province, College of Agricultural Sciences, Guizhou University, Guiyang, 550025, China. zmfang@gzu.edu.cn.
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), Guizhou University, Guiyang, 550025, China. zmfang@gzu.edu.cn.
Publications dans "Systèmes de transport d'acides aminés neutres" :
Department of DiBEST (Biologia, Ecologia, Scienze Della Terra) Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Via Bucci 4C, 87036, Arcavacata Di Rende, CS, Italy. cesare.indiveri@unical.it.
CNR Institute of Biomembranes, Bioenergetics and Molecular Biotechnologies (IBIOM), Via Amendola 122/O, 70126, Bari, Italy. cesare.indiveri@unical.it.
Publications dans "Systèmes de transport d'acides aminés neutres" :
Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain; ekaitz.errasti@irbbarcelona.org ollorca@cnio.es manuel.palacin@irbbarcelona.org.
Department of Biochemistry and Molecular Biomedicine, Universitat de Barcelona, E-08028 Barcelona, Spain.
Publications dans "Systèmes de transport d'acides aminés neutres" :
Structural Biology Programme, Spanish National Cancer Research Centre, E-28029 Madrid, Spain; ekaitz.errasti@irbbarcelona.org ollorca@cnio.es manuel.palacin@irbbarcelona.org.
Publications dans "Systèmes de transport d'acides aminés neutres" :
Institute for Research in Biomedicine (IRB Barcelona), The Barcelona Institute of Science and Technology (BIST), 08028 Barcelona, Spain; ekaitz.errasti@irbbarcelona.org ollorca@cnio.es manuel.palacin@irbbarcelona.org.
Department of Biochemistry and Molecular Biomedicine, Universitat de Barcelona, E-08028 Barcelona, Spain.
U731, Centro de Investigación Biomedica En Red de Enfermedades Raras (CIBERER), 08028 Barcelona, Spain.
Publications dans "Systèmes de transport d'acides aminés neutres" :
Institute of Rice Industry Technology Research, Key Laboratory of Functional Agriculture of Guizhou Provincial, Department of Education, Key Laboratory of Molecular Breeding for Grain and oil Crops in Guizhou Province, College of Agricultural Sciences, Guizhou University, Guiyang, 550025, China.
Publications dans "Systèmes de transport d'acides aminés neutres" :
Membrane Enzymology, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, Groningen, The Netherlands. d.j.slotboom@rug.nl.
University of Groningen, Zernike Institute for Advanced Materials, Groningen, The Netherlands. d.j.slotboom@rug.nl.
Publications dans "Systèmes de transport d'acides aminés neutres" :
Membrane Enzymology, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, Groningen, The Netherlands. c.paulino@rug.nl.
Structural Biology, University of Groningen, Groningen Biomolecular Sciences and Biotechnology Institute, Groningen, The Netherlands. c.paulino@rug.nl.
Publications dans "Systèmes de transport d'acides aminés neutres" :
State Key Laboratory of Genetic Engineering, Department of Genetics, School of Life Sciences, Fudan University, Shanghai, China.
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Agricultural Sciences, Guizhou University, Guiyang, China.
Publications dans "Systèmes de transport d'acides aminés neutres" :
Department DiBEST (Biologia, Ecologia, Scienze della Terra), Unit of Biochemistry and Molecular Biotechnology, University of Calabria, Arcavacata, Italy.
Publications dans "Systèmes de transport d'acides aminés neutres" :
Areca palm velarivirus 1 (APV1) is one of the main pathogen causing yellow leaf disease, and leading to considerable losses in the Areca palm industry. The detection methods for APV1 are primarily bas...
The aim of this study was to evaluate the time in days between symptom onset and first positive real-time reverse transcriptase polymerase chain reaction (RT-PCR) result for COVID-19....
This systematic review was conducted in the MEDLINE (PubMed), Embase, and Scopus databases using the following descriptors: "COVID-19", "SARS-CoV-2", "coronavirus", "RT-PCR", "real time PCR", and "dia...
The included studies were conducted in 31 different countries and reported on a total of 6831 patients. The median age of the participants was 49.95 years. The three most common symptoms were fever, c...
These findings corroborate the RT-PCR COVID-19 testing practices of some health units. In addition, the most frequently described symptoms of these patients can be considered the initial symptoms of i...
Quantitative polymerase chain reaction (qPCR) offers the capabilities of real-time monitoring of amplified products, fast detection, and quantitation of infectious units, but poses technical hurdles f...
During the COVID-19 pandemic, the reverse transcriptase-quantitative polymerase chain reaction (RT-qPCR) assay has been the primary method of diagnosis of SARS-CoV-2 infection. However, RT-qPCR assay ...
Many countries have applied the wastewater surveillance of the COVID-19 pandemic to their national public health monitoring measures. The most used methods for detecting SARS-CoV-2 in wastewater are q...
This study aims to compare RT-qPCR and RT-ddPCR for detecting SARS-CoV-2 in wastewater. It also aimed to investigate the effect of changes in the analytical pipeline, including the RNA extraction kit,...
We compared 2 RT-qPCR kits, TaqMan RT-qPCR and QuantiTect RT-qPCR, and RT-ddPCR based on sensitivity, positivity rates, variability, and correlation of SARS-CoV-2 gene copy numbers in wastewater to th...
Our results indicated that the most sensitive method to detect SARS-CoV-2 in wastewater was RT-ddPCR. It had the highest positivity rate (26/30), and its limit of detection was the lowest (0.06 gene c...
As our study, as well as most of the previous studies, has shown RT-ddPCR to be more sensitive than RT-qPCR, its use in the wastewater surveillance of SARS-CoV-2 should be considered, especially if th...
In order to rapidly differentiate sublineages BA.1 and BA.2 of the SARS-CoV-2 variant of concern Omicron, we developed a real-time reverse-transcriptase polymerase chain reaction to target the discrim...
Existing real-time reverse transcriptase PCR (RT-qPCR) has certain limitations for the point-of-care detection of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) since it requires sophist...
The RT-PSR assay was optimized using RdRp gene and evaluated for the detection of SARS-CoV-2. The time of 60min and a temperature of 63°C was optimized for targeting the RNA-dependent RNA polymerase g...
The specific primers designed for this assay showed 100% specificity and did not react when tested with other lung infection-causing viruses and bacteria. The optimized assay was validated with 190 cl...
The RT-PSR assay can be considered for rapid and sensitive detection of SARS-CoV-2, particularly in resource-limited settings. To our knowledge, there is as yet no RT-PSR-based kit developed for SARS-...
COVID-19 pandemic highlighted the demand for the fast and reliable detection of viral RNA. Although various methods for RNA amplification and detection have been proposed, some limitations, including ...
Although conventional polymerase chain reaction (PCR) methods are widely used in diagnosis, the titer of the pathogenic virus is difficult to determine based on the PCR. In our prior report, a long-ra...
IAV was inactivated by: heating at 100 °C for periods ranging from 1 to 15 min, treating with 0.12% sodium hypochlorite for periods ranging from 3 to 30 min, or treating with 70% ethanol for periods r...
One minute heating, 3 min sodium hypochlorite treatment, or 10 min ethanol treatment was sufficient to deactivate IAV. Changes before and after the inactivations in the copy numbers on LR-RT-qPCR were...
In addition to good applicability in UV-irradiation previously reported, the LR-RT-qPCR method is suitable for evaluating the effect of heat-inactivation on IAV infectivity. However, minor modificatio...
Sapovirus (SaV) is a causative agent of human gastroenteritis in both community outbreaks and sporadic cases worldwide. Shellfish accumulate a variety of pathogens during filter feeding. In particular...