Quorum-dependent transfer of the opine-catabolic plasmid pAoF64/95 is regulated by a novel mechanism involving inhibition of the TraR antiactivator TraM.
agrobacterium
bacterial plasmids
gene regulation
quorum-sensing
Journal
MicrobiologyOpen
ISSN: 2045-8827
Titre abrégé: Microbiologyopen
Pays: England
ID NLM: 101588314
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
25
09
2017
revised:
27
01
2018
accepted:
30
01
2018
pubmed:
11
4
2018
medline:
5
3
2019
entrez:
11
4
2018
Statut:
ppublish
Résumé
We previously described a plasmid of Agrobacterium spp., pAoF64/95, in which the quorum-sensing system that controls conjugative transfer is induced by the opine mannopine. We also showed that the quorum-sensing regulators TraR, TraM, and TraI function similarly to their counterparts in other repABC plasmids. However, traR, unlike its counterpart on Ti plasmids, is monocistronic and not located in an operon that is inducible by the conjugative opine. Here, we report that both traR and traM are expressed constitutively and not regulated by growth with mannopine. We report two additional regulatory genes, mrtR and tmsP, that are involved in a novel mechanism of control of TraR activity. Both genes are located in the distantly linked region of pAoF64/95 encoding mannopine utilization. MrtR, in the absence of mannopine, represses the four-gene mocC operon as well as tmsP, which is the distal gene of the eight-gene motA operon. As judged by a bacterial two-hybrid analysis, TmsP, which shows amino acid sequence relatedness with the TraM-binding domain of TraR, interacts with the antiactivator. We propose a model in which mannopine, acting through the repressor MrtR, induces expression of TmsP which then titrates the levels of TraM thereby freeing TraR to activate the tra regulon.
Identifiants
pubmed: 29635848
doi: 10.1002/mbo3.625
pmc: PMC6341043
doi:
Substances chimiques
Mannitol
3OWL53L36A
mannopine
87084-52-4
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.
Langues
eng
Sous-ensembles de citation
IM
Pagination
e00625Subventions
Organisme : NIGMS NIH HHS
ID : R01 GM052465
Pays : United States
Informations de copyright
© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
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