Identification of genes related to chlamydospore formation in Clonostachys rosea 67-1.
Clonostachys rosea
chlamydospore
differentially expressed gene
reverse transcription quantitative PCR
transcriptome
Journal
MicrobiologyOpen
ISSN: 2045-8827
Titre abrégé: Microbiologyopen
Pays: England
ID NLM: 101588314
Informations de publication
Date de publication:
01 2019
01 2019
Historique:
received:
06
12
2017
revised:
11
02
2018
accepted:
16
02
2018
pubmed:
11
4
2018
medline:
5
3
2019
entrez:
11
4
2018
Statut:
ppublish
Résumé
Chlamydospores are specific structures that are of great significance to the commercialization of fungal biopesticides. To explore the genes associated with chlamydospore formation, a biocontrol fungus Clonostachys rosea 67-1 that is capable of producing resistant spores under particular conditions was investigated by transcriptome sequencing and analysis. A total of 549,661,174 clean reads were obtained, and a series of differentially expressed genes potentially involved in fungal chlamydospore formation were identified. At 36 hr, 67 and 117 genes were up- and downregulated in C. rosea during chlamydospore production, compared with the control for conidiation, and 53 and 24 genes were up- and downregulated at 72 hr. GO classification suggested that the differentially expressed genes were related to cellular component, biological process, and molecular function categories. A total of 188 metabolism pathways were linked to chlamydospore production by KEGG analysis. Sixteen differentially expressed genes were verified by reverse transcription quantitative PCR, and the expression profiles were consistent with the transcriptome data. To the best of our knowledge, it is the first report on the genes associated with chlamydospore formation in C. rosea. The results provide insight into the molecular mechanisms underlying C. rosea sporulation, which will assist the development of fungal biocontrol agents.
Identifiants
pubmed: 29635882
doi: 10.1002/mbo3.624
pmc: PMC6341034
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e00624Informations de copyright
© 2018 The Authors. MicrobiologyOpen published by John Wiley & Sons Ltd.
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