Natural variation of ZmHKT1 affects root morphology in maize at the seedling stage.
Cation Transport Proteins
/ genetics
Genetic Association Studies
Genetic Variation
Plant Proteins
/ genetics
Plant Roots
/ anatomy & histology
Polymorphism, Single Nucleotide
/ genetics
Quantitative Trait, Heritable
Seedlings
/ anatomy & histology
Sequence Analysis, DNA
Zea mays
/ anatomy & histology
Gene-based association mapping
Maize
Nucleotide diversity
Root traits
ZmHKT1 gene
Journal
Planta
ISSN: 1432-2048
Titre abrégé: Planta
Pays: Germany
ID NLM: 1250576
Informations de publication
Date de publication:
Mar 2019
Mar 2019
Historique:
received:
26
07
2018
accepted:
12
11
2018
pubmed:
22
11
2018
medline:
12
3
2019
entrez:
22
11
2018
Statut:
ppublish
Résumé
Eight variants in ZmHKT1 promoter were significantly associated with root diameter, four haplotypes based on these significant variants were found, and Hap2 has the largest root diameter. Roots play an important role in uptake of water, nutrients and plant anchorage. Identification of gene and corresponding SNPs associated with root traits would enable develop maize lines with better root traits that might help to improve capacity for absorbing nutrients and water acquisition. The genomic sequences of a salt tolerance gene ZmHKT1 was resequenced in 349 maize inbred lines, and the association between nucleotide polymorphisms and seedling root traits was detected. A total of 269 variants in ZmHKT1 were identified, including 226 single nucleotide polymorphisms and 43 insertions and deletions. The gene displayed high level of nucleotide diversity, especially in non-genic regions. A total of 19 variations in untranslated region of ZmHKT1 were found to be associated with six seedling traits. Eight variants in promoter region were significantly associated with average root diameter (ARD), four haplotypes were found based on these significant variants, and Hap2 has the largest ARD. Two SNPs in high-linkage disequilibrium (SNP-415 and SNP 2169) with pleiotropic effects were significantly associated with plant height, root surface area, root volume, and shoot dry weight. This result revealed that ZmHKT1 was an important contributor to the phenotypic variations of seedling root traits in maize, these significant variants could use to develop functional markers to improve root traits.
Identifiants
pubmed: 30460404
doi: 10.1007/s00425-018-3043-2
pii: 10.1007/s00425-018-3043-2
doi:
Substances chimiques
Cation Transport Proteins
0
Plant Proteins
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
879-889Subventions
Organisme : National Natural Science Foundations
ID : 31601810
Organisme : National Key Technology Research and Development Program of MOST
ID : 2016YFD0100303
Organisme : Natural Science Foundations of Jiangsu Province
ID : BK20150010
Organisme : Innovative Research Team of Universities in Jiangsu Province
ID : no
Organisme : Project funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
ID : no
Organisme : China Postdoctoral Science Foundation
ID : 2016M591936
Organisme : China Postdoctoral Science Foundation
ID : 2018T110563
Organisme : Jiangsu Planned Projects for Postdoctoral Research Funds
ID : 1601131B
Organisme : Qing-Lan Project of Jiangsu Province
ID : no
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