Population genomics demystifies the defoliation phenotype in the plant pathogen Verticillium dahliae.
Verticillium dahliae
N-acylethanolamines (NAEs)
defoliating phenotype
lineage-specific genes
secondary metabolites
Journal
The New phytologist
ISSN: 1469-8137
Titre abrégé: New Phytol
Pays: England
ID NLM: 9882884
Informations de publication
Date de publication:
04 2019
04 2019
Historique:
received:
02
07
2018
accepted:
18
12
2018
pubmed:
5
1
2019
medline:
28
2
2020
entrez:
5
1
2019
Statut:
ppublish
Résumé
Verticillium dahliae is a broad host-range pathogen that causes vascular wilts in plants. Interactions between three hosts and specific V. dahliae genotypes result in severe defoliation. The underlying mechanisms of defoliation are unresolved. Genome resequencing, gene deletion and complementation, gene expression analysis, sequence divergence, defoliating phenotype identification, virulence analysis, and quantification of V. dahliae secondary metabolites were performed. Population genomics previously revealed that G-LSR2 was horizontally transferred from the fungus Fusarium oxysporum f. sp. vasinfectum to V. dahliae and is exclusively found in the genomes of defoliating (D) strains. Deletion of seven genes within G-LSR2, designated as VdDf genes, produced the nondefoliation phenotype on cotton, olive, and okra but complementation of two genes restored the defoliation phenotype. Genes VdDf5 and VdDf6 associated with defoliation shared homology with polyketide synthases involved in secondary metabolism, whereas VdDf7 shared homology with proteins involved in the biosynthesis of N-lauroylethanolamine (N-acylethanolamine (NAE) 12:0), a compound that induces defoliation. NAE overbiosynthesis by D strains also appears to disrupt NAE metabolism in cotton by inducing overexpression of fatty acid amide hydrolase. The VdDfs modulate the synthesis and overproduction of secondary metabolites, such as NAE 12:0, that cause defoliation either by altering abscisic acid sensitivity, hormone disruption, or sensitivity to the pathogen.
Identifiants
pubmed: 30609067
doi: 10.1111/nph.15672
pmc: PMC6594092
doi:
Substances chimiques
Ethanolamines
0
Lauric Acids
0
N-lauroylethanolamine
142-78-9
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1012-1029Subventions
Organisme : National Key Research and Development Program of China
ID : 2017YFD0201900
Pays : International
Organisme : National Key Research and Development Program of China
ID : 2017YFD0200601
Pays : International
Organisme : Fundamental Research Funds for Central Non-profit Scientific Institution
ID : Y2016CG11
Pays : International
Organisme : Fundamental Research Funds for Central Non-profit Scientific Institution
ID : S2016JC05
Pays : International
Organisme : Fundamental Research Funds for Central Non-profit Scientific Institution
ID : S2016CG01
Pays : International
Organisme : Young Elite Scientists Sponsorship Program
ID : 2016QNRC001
Pays : International
Organisme : Special Public Welfare Industry Research on Agriculture
ID : 201503109
Pays : International
Organisme : CAAS (an Agricultural Science and Technology Innovation Program grant to X.F.D)
Pays : International
Organisme : NSFC
ID : 31870138
Pays : International
Organisme : NSFC
ID : 31671986
Pays : International
Organisme : NSFC
ID : 31772245
Pays : International
Organisme : NSFC
ID : 31501600
Pays : International
Informations de copyright
© 2019 The Authors. New Phytologist © 2019 New Phytologist Trust.
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