Proteomic Analysis of Histones H2A/H2B and Variant Hv1 in Tetrahymena thermophila Reveals an Ancient Network of Chaperones.

Amino Acid Sequence Evolution, Molecular Histone Chaperones / metabolism Histones / metabolism Phylogeny Poly(ADP-ribose) Polymerases / metabolism Proteome Proteomics Tetrahymena thermophila / metabolism
FACT histone histone H2A histone H2B histone chaperone

Journal

Molecular biology and evolution
ISSN: 1537-1719
Titre abrégé: Mol Biol Evol
Pays: United States
ID NLM: 8501455

Informations de publication

Date de publication:
01 05 2019
Historique:
pubmed: 24 2 2019
medline: 2 7 2019
entrez: 24 2 2019
Statut: ppublish

Résumé

Epigenetic information, which can be passed on independently of the DNA sequence, is stored in part in the form of histone posttranslational modifications and specific histone variants. Although complexes necessary for deposition have been identified for canonical and variant histones, information regarding the chromatin assembly pathways outside of the Opisthokonts remains limited. Tetrahymena thermophila, a ciliated protozoan, is particularly suitable to study and unravel the chromatin regulatory layers due to its unique physical separation of chromatin states in the form of two distinct nuclei present within the same cell. Using a functional proteomics pipeline, we carried out affinity purification followed by mass spectrometry of endogenously tagged T. thermophila histones H2A, H2B and variant Hv1.We identified a set of interacting proteins shared among the three analyzed histones that includes the FACT-complex, as well as H2A- or Hv1-specific chaperones. We find that putative subunits of T. thermophila versions of SWR- and INO80-complexes, as well as transcription-related histone chaperone Spt6Tt specifically copurify with Hv1. We also identified importin β6 and the T. thermophila ortholog of nucleoplasmin 1 (cNpl1Tt) as H2A-H2B interacting partners. Our results further implicate Poly [ADP-ribose] polymerases in histone metabolism. Molecular evolutionary analysis, reciprocal affinity purification coupled to mass spectrometry experiments, and indirect immunofluorescence studies using endogenously tagged Spt16Tt (FACT-complex subunit), cNpl1Tt, and PARP6Tt underscore the validity of our approach and offer mechanistic insights. Our results reveal a highly conserved regulatory network for H2A (Hv1)-H2B concerning their nuclear import and assembly into chromatin.

Identifiants

DOI: 10.1093/molbev/msz039 PMID: 30796450 PMC: PMC6502085
pubmed: 30796450
pii: 5362030
doi: 10.1093/molbev/msz039
pmc: PMC6502085
doi:

Substances chimiques

Histone Chaperones 0
Histones 0
Proteome 0
Poly(ADP-ribose) Polymerases EC 2.4.2.30

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

1037-1055

Informations de copyright

© The Author(s) 2019. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

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Auteurs

Kanwal Ashraf (K)

Department of Biology, York University, Toronto, ON, Canada.

Syed Nabeel-Shah (S)

Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada.
Donnelly Centre, University of Toronto, Toronto, ON, Canada; Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.

Jyoti Garg (J)

Department of Biology, York University, Toronto, ON, Canada.

Alejandro Saettone (A)

Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada.

Joanna Derynck (J)

Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada.

Anne-Claude Gingras (AC)

Department of Molecular Genetics, University of Toronto, Toronto, ON, Canada.
Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, ON, Canada.

Jean-Philippe Lambert (JP)

Department of Molecular Medicine and Cancer Research Centre, Université Laval, Québec, QC, Canada.
CHU de Québec Research Center, CHUL, Québec, QC, Canada.

Ronald E Pearlman (RE)

Department of Biology, York University, Toronto, ON, Canada.

Jeffrey Fillingham (J)

Department of Chemistry and Biology, Ryerson University, Toronto, ON, Canada.

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Classifications MeSH

questionsmedicales.fr › Sciences de l'information › Sources d'information › Services d'information › Documentation › Données de séquences moléculaires › Séquence d'acides aminés › Proteomic Analysis of Histones H2A/H2B and...
questionsmedicales.fr › Phénomènes biologiques › Évolution biologique › Évolution moléculaire › Proteomic Analysis of Histones H2A/H2B and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Chaperons moléculaires › Chaperons d'histones › Proteomic Analysis of Histones H2A/H2B and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Nucléoprotéines › Histone › Proteomic Analysis of Histones H2A/H2B and...
questionsmedicales.fr › Sciences de l'information › Phylogenèse › Proteomic Analysis of Histones H2A/H2B and...
questionsmedicales.fr › Enzymes et coenzymes › Enzymes › Transferases › Glycosyltransferase › Pentosyltransferases › ADP ribose transferases › Poly(ADP-ribose) polymerases › Proteomic Analysis of Histones H2A/H2B and...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéome › Proteomic Analysis of Histones H2A/H2B and...
questionsmedicales.fr › Disciplines des sciences naturelles › Chimie › Biochimie › Protéomique › Proteomic Analysis of Histones H2A/H2B and...
questionsmedicales.fr › Eucaryotes › Alveolata › Ciliophora › Oligohymenophorea › Hymenostomatida › Tetrahymenina › Tetrahymena › Tetrahymena thermophila › Proteomic Analysis of Histones H2A/H2B and...