Phylogeny-guided characterization of glycosyltransferases for epothilone glycosylation.
Journal
Microbial biotechnology
ISSN: 1751-7915
Titre abrégé: Microb Biotechnol
Pays: United States
ID NLM: 101316335
Informations de publication
Date de publication:
07 2019
07 2019
Historique:
received:
19
01
2019
revised:
05
04
2019
accepted:
16
04
2019
pubmed:
10
5
2019
medline:
16
4
2020
entrez:
10
5
2019
Statut:
ppublish
Résumé
Glycosylation of natural products can influence their pharmacological properties, and efficient glycosyltransferases (GTs) are critical for this purpose. The polyketide epothilones are potent anti-tumour compounds, and YjiC is the only reported GT for the glycosylation of epothilone. In this study, we phylogenetically analysed 8261 GTs deposited in CAZy database and revealed that YjiC locates in a subbranch of the Macrolide I group, forming the YjiC-subbranch with 160 GT sequences. We demonstrated that the YjiC-subbranch GTs are normally efficient in epothilone glycosylation, but some showed low glycosylation activities. Sequence alignment of YjiC-subbranch showed that the 66th and 77th amino acid residues, which were close to the catalytic cavity in molecular docking model, were conserved in five high-active GTs (Q66 and P77) but changed in two low-efficient GTs. Site-directed residues swapping at the two positions in the two low-active GTs (BssGT and BamGT) and the high-active GT BsGT-1 demonstrated that the two amino acid residues played an important role in the catalytic efficiency of epothilone glycosylation. This study highlights that the potent GTs for appointed compounds are phylogenetically grouped with conserved residues for the catalytic efficiency.
Identifiants
pubmed: 31069998
doi: 10.1111/1751-7915.13421
pmc: PMC6559208
doi:
Substances chimiques
Epothilones
0
Tubulin Modulators
0
Glycosyltransferases
EC 2.4.-
Banques de données
GENBANK
['AAU40842', 'CUB50191', 'ADP31706', 'SCA85980', 'ARA85718', 'AMA51908', 'AKD21753', 'NP389824', 'AAS41089', 'WP003234124']
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
763-774Informations de copyright
© 2019 The Authors. Microbial Biotechnology published by John Wiley & Sons Ltd and Society for Applied Microbiology.
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