Cytogenetic and molecular genotyping in the allotetraploid Festuca pratensis × Lolium perenne hybrids.
Festuca
Festulolium hybrids
Genomic composition
Inheritance pattern
Lolium
Rearrangements
Recombination
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
14 May 2019
14 May 2019
Historique:
received:
25
10
2018
accepted:
03
05
2019
entrez:
16
5
2019
pubmed:
16
5
2019
medline:
5
9
2019
Statut:
epublish
Résumé
Species of the Festuca and Lolium genera, as well as intergeneric Festuca × Lolium (Festulolium) hybrids, are valuable fodder and turf grasses for agricultural and amenity purposes worldwide. Festulolium hybrids can merge in their genomes agronomically important characteristics. However, in polyploid plants, especially in allopolyploids, the hybridization of divergent genomes could contribute to various abnormalities, such as variability in chromosome number, structural rearrangements, and/or disorders in inheritance patterns. Here we studied these issues in allotetraploid Festuca pratensis × Lolium perenne hybrids. Cytogenetic procedures, including fluorescent in situ hybridization, genomic in situ hybridization, and molecular markers - inter-simple sequence repeats (ISSR) were exploited. This cytogenetic approach indicated the dynamics in the number and distribution of ribosomal RNA genes and structural rearrangements for both parental genomes (Festuca and Lolium) in hybrid karyotypes. The separate analysis of F. pratensis and L. perenne chromosomes in hybrid plants (F Our study reports cytogenetic and molecular genotyping of the F. pratensis × L. perenne hybrid and its following F
Sections du résumé
BACKGROUND
BACKGROUND
Species of the Festuca and Lolium genera, as well as intergeneric Festuca × Lolium (Festulolium) hybrids, are valuable fodder and turf grasses for agricultural and amenity purposes worldwide. Festulolium hybrids can merge in their genomes agronomically important characteristics. However, in polyploid plants, especially in allopolyploids, the hybridization of divergent genomes could contribute to various abnormalities, such as variability in chromosome number, structural rearrangements, and/or disorders in inheritance patterns. Here we studied these issues in allotetraploid Festuca pratensis × Lolium perenne hybrids.
RESULTS
RESULTS
Cytogenetic procedures, including fluorescent in situ hybridization, genomic in situ hybridization, and molecular markers - inter-simple sequence repeats (ISSR) were exploited. This cytogenetic approach indicated the dynamics in the number and distribution of ribosomal RNA genes and structural rearrangements for both parental genomes (Festuca and Lolium) in hybrid karyotypes. The separate analysis of F. pratensis and L. perenne chromosomes in hybrid plants (F
CONCLUSION
CONCLUSIONS
Our study reports cytogenetic and molecular genotyping of the F. pratensis × L. perenne hybrid and its following F
Identifiants
pubmed: 31088367
doi: 10.1186/s12864-019-5766-2
pii: 10.1186/s12864-019-5766-2
pmc: PMC6518686
doi:
Substances chimiques
DNA, Ribosomal
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
367Subventions
Organisme : Narodowe Centrum Nauki
ID : 2018/28/T/NZ9/00074
Références
Nucleic Acids Res. 1980 Nov 11;8(21):4851-65
pubmed: 7443527
Theor Appl Genet. 1985 Dec;71(2):185-92
pubmed: 24247381
BMC Plant Biol. 2011 Sep 12;11:126
pubmed: 21910890
Ann Bot. 2012 Dec;110(8):1593-606
pubmed: 23087127
BMC Evol Biol. 2013 Jul 03;13:136
pubmed: 23819574
Theor Appl Genet. 2006 Mar;112(5):924-33
pubmed: 16397788
Trends Genet. 2012 Jan;28(1):22-32
pubmed: 22094264
Theor Appl Genet. 2005 Jan;110(2):323-36
pubmed: 15558229
Science. 2008 Apr 25;320(5875):481-3
pubmed: 18436776
Proc Natl Acad Sci U S A. 2013 Feb 26;110(9):3447-52
pubmed: 23401544
Nucleic Acids Res. 1990 Jul 11;18(13):4011
pubmed: 2100998
Genetics. 1994 Dec;138(4):1251-74
pubmed: 7896104
Proc Natl Acad Sci U S A. 2000 Jun 6;97(12):6608-13
pubmed: 10823890
Rice (N Y). 2014 Dec;7(1):3
pubmed: 26055992
Chromosoma. 2000 Jun;109(3):161-72
pubmed: 10929194
Plant Mol Biol. 2000 Jan;42(1):225-49
pubmed: 10688139
Methods Mol Biol. 2011;701:315-26
pubmed: 21181539
Heredity (Edinb). 2013 May;110(5):475-83
pubmed: 23321705
PLoS One. 2012;7(5):e36963
pubmed: 22606317
Chromosome Res. 2008;16(4):575-85
pubmed: 18409011
G3 (Bethesda). 2015 May 26;5(8):1585-92
pubmed: 26019188
Heredity (Edinb). 2006 Mar;96(3):243-51
pubmed: 16449983
Genome. 1996 Jun;39(3):535-42
pubmed: 18469914
Plant Cell. 2001 Aug;13(8):1749-59
pubmed: 11487690
Genome Res. 2010 Nov;20(11):1545-57
pubmed: 20876790
Heredity (Edinb). 1999 Sep;83 ( Pt 3):298-303
pubmed: 10504427
Plant Genome. 2015 Mar;8(1):eplantgenome2014.07.0032
pubmed: 33228282
Genetics. 2006 Mar;172(3):1939-53
pubmed: 16322527
Theor Appl Genet. 2006 Aug;113(4):731-42
pubmed: 16832647
Theor Appl Genet. 2006 Aug;113(3):539-47
pubmed: 16773330
Appl Plant Sci. 2018 Jun 24;6(6):e01159
pubmed: 30131901
Genome. 2007 Oct;50(10):907-26
pubmed: 18059554
Theor Appl Genet. 1999 Aug;99(3-4):445-52
pubmed: 22665177
Genetics. 2005 Jan;169(1):347-53
pubmed: 15466426
Cytogenet Genome Res. 2010 Jul;129(1-3):162-72
pubmed: 20501979
Breed Sci. 2016 Mar;66(2):234-43
pubmed: 27162495
PLoS One. 2017 Jun 7;12(6):e0179043
pubmed: 28591168
Plant Cell. 2001 Aug;13(8):1735-47
pubmed: 11487689
Plant J. 2005 Jan;41(2):221-30
pubmed: 15634199
Nat Rev Cancer. 2017 Jul 25;17(8):489-501
pubmed: 28740117
Proc Natl Acad Sci U S A. 2004 Dec 28;101(52):18240-5
pubmed: 15604143
Genome. 1994 Apr;37(2):181-9
pubmed: 18470069
Cytogenet Genome Res. 2005;110(1-4):91-107
pubmed: 16093661
Theor Appl Genet. 2005 Feb;110(3):527-36
pubmed: 15619078
Theor Appl Genet. 1994 Sep;88(8):909-13
pubmed: 24186241
Protoplasma. 2015 Jul;252(4):985-96
pubmed: 25480732
Nature. 1975 Apr 17;254(5501):595-7
pubmed: 1128654
PLoS One. 2012;7(2):e31033
pubmed: 22363542
Proc Natl Acad Sci U S A. 2004 Oct 12;101(41):14818-23
pubmed: 15466712
PLoS One. 2013 Nov 15;8(11):e79329
pubmed: 24260197
BMC Genomics. 2009 Oct 15;10:473
pubmed: 19832973
PLoS One. 2008 Jan 02;3(1):e1402
pubmed: 18167561
Heredity (Edinb). 2013 Jan;110(1):63-70
pubmed: 23047202
Genome. 1997 Jun;40(3):406-10
pubmed: 18464836
Am J Bot. 2016 Jul;103(7):1167-74
pubmed: 27425631
Proc Natl Acad Sci U S A. 1938 Sep;24(9):372-7
pubmed: 16588241
Genetics. 2005 Feb;169(2):967-79
pubmed: 15520255
Protoplasma. 2018 Mar;255(2):451-458
pubmed: 28884345
PLoS One. 2008 May 14;3(5):e2167
pubmed: 18478113