Biocontrol effect of Kluyveromyces lactis on aflatoxin expression and production in Aspergillus parasiticus.
Aspergillus parasiticus
Kluyveromyces lactis
aflR gene
aflatoxin
Journal
FEMS microbiology letters
ISSN: 1574-6968
Titre abrégé: FEMS Microbiol Lett
Pays: England
ID NLM: 7705721
Informations de publication
Date de publication:
01 05 2019
01 05 2019
Historique:
received:
16
03
2018
accepted:
25
05
2019
pubmed:
28
5
2019
medline:
27
6
2020
entrez:
28
5
2019
Statut:
ppublish
Résumé
Aspergillus parasiticus is one of the most common fungi able to produce aflatoxins, which are naturally occurring carcinogenic substances. This study evaluated the effects of the safe yeast, Kluyveromyces lactis, on fungal growth, aflatoxin production and expression of aflR gene in A. parasiticus. Antifungal susceptibility was evaluated by exposing A. parasiticus to different amounts of K. lactis, and aflatoxin production was measured using high-performance liquid chromatography. Expression of the aflR gene was determined by measuring the cognate aflR mRNA level by quantitative real-time reverse-transcription polymerase chain reaction assay. The growth of A. parasiticus was inhibited by 7 days of incubation at 30°C with a minimum population of 1.5 × 105 CFU/ml of K. lactis, which also suppressed expression of the A. parasiticus aflR gene, reducing the total production of aflatoxins by 97.9% and aflatoxins B1, B2, G1 and G2 by 97.8, 98.6, 98 and 94%, respectively. Accordingly, K. lactis could be considered as a potential biocontrol agent against toxigenic molds in food and animal feed.
Identifiants
pubmed: 31132114
pii: 5499020
doi: 10.1093/femsle/fnz114
pii:
doi:
Substances chimiques
AFLR protein, Aspergillus
0
Aflatoxins
0
Biological Control Agents
0
DNA-Binding Proteins
0
Fungal Proteins
0
Transcription Factors
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© FEMS 2019.