A novel double-stranded RNA mycovirus isolated from Trichoderma harzianum.
Mycovirus
Trichoderma harzianum
Trichoderma harzianum mycovirus 1
dsRNA
Journal
Virology journal
ISSN: 1743-422X
Titre abrégé: Virol J
Pays: England
ID NLM: 101231645
Informations de publication
Date de publication:
11 09 2019
11 09 2019
Historique:
received:
01
12
2018
accepted:
05
08
2019
entrez:
13
9
2019
pubmed:
13
9
2019
medline:
20
12
2019
Statut:
epublish
Résumé
Trichoderma spp. are used extensively in agriculture as biological control agents to prevent soil-borne plant diseases. In recent years, mycoviruses from fungi have attracted increasing attention due to their effects on their hosts, but Trichoderma mycoviruses have not been the subject of extensive study. We sought to discover novel mycoviruses from Trichoderma spp. and to determine the effects of the biocontrol function of Trichoderma spp. Mycoviruses were screened by dsRNA extraction and metagenomic analysis. RT-PCR, 5' RACE, and 3' RACE were used to obtain the genome sequence. MEGA software was used to classify the new mycovirus. The effects of the identified mycovirus on the biological properties of the host strain 525 were evaluated using cucumber plants and Fusarium oxysporum f. sp. cucumerinum. A novel mycovirus, Trichoderma harzianum mycovirus 1 (ThMV1) (accession number MH155602), was discovered in Trichoderma harzianum strain 525, a soil-borne fungus collected from Inner Mongolia, China. The mycovirus exhibited a double-stranded RNA (dsRNA) genome with a complete genome sequence of 3160 base pairs and two open reading frames (ORFs) on the negative strand. Phylogenetic analysis indicated that it belongs to an unclassified family of dsRNA mycoviruses. The removal of ThMV1 from the host 525 strain reduced host biomass production and improved the biocontrol capability of the host for Fusarium oxysporum f. sp. cucumerinum. At same time, the presence of ThMV1 improved the growth of cucumber. ThMV1 is a new unclassified mycovirus found in T. harzianum. It not only affects the phenotype of the host strain but also reduces its biocontrol function, which sheds light on the interaction between the mycovirus and Trichoderma spp.
Sections du résumé
BACKGROUND
Trichoderma spp. are used extensively in agriculture as biological control agents to prevent soil-borne plant diseases. In recent years, mycoviruses from fungi have attracted increasing attention due to their effects on their hosts, but Trichoderma mycoviruses have not been the subject of extensive study. We sought to discover novel mycoviruses from Trichoderma spp. and to determine the effects of the biocontrol function of Trichoderma spp.
METHODS
Mycoviruses were screened by dsRNA extraction and metagenomic analysis. RT-PCR, 5' RACE, and 3' RACE were used to obtain the genome sequence. MEGA software was used to classify the new mycovirus. The effects of the identified mycovirus on the biological properties of the host strain 525 were evaluated using cucumber plants and Fusarium oxysporum f. sp. cucumerinum.
RESULTS
A novel mycovirus, Trichoderma harzianum mycovirus 1 (ThMV1) (accession number MH155602), was discovered in Trichoderma harzianum strain 525, a soil-borne fungus collected from Inner Mongolia, China. The mycovirus exhibited a double-stranded RNA (dsRNA) genome with a complete genome sequence of 3160 base pairs and two open reading frames (ORFs) on the negative strand. Phylogenetic analysis indicated that it belongs to an unclassified family of dsRNA mycoviruses. The removal of ThMV1 from the host 525 strain reduced host biomass production and improved the biocontrol capability of the host for Fusarium oxysporum f. sp. cucumerinum. At same time, the presence of ThMV1 improved the growth of cucumber.
CONCLUSION
ThMV1 is a new unclassified mycovirus found in T. harzianum. It not only affects the phenotype of the host strain but also reduces its biocontrol function, which sheds light on the interaction between the mycovirus and Trichoderma spp.
Identifiants
pubmed: 31511029
doi: 10.1186/s12985-019-1213-x
pii: 10.1186/s12985-019-1213-x
pmc: PMC6737671
doi:
Substances chimiques
Biological Control Agents
0
RNA, Double-Stranded
0
RNA, Viral
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
113Références
Virus Res. 2016 Jul 2;219:22-38
pubmed: 26546154
Nat Rev Microbiol. 2006 Mar;4(3):212-21
pubmed: 16489348
Mycol Res. 2003 Jul;107(Pt 7):847-53
pubmed: 12967212
Virus Res. 2014 Aug 8;188:128-41
pubmed: 24768846
Annu Rev Phytopathol. 2004;42:311-38
pubmed: 15283669
J Gen Virol. 2009 Jun;90(Pt 6):1423-1432
pubmed: 19264638
FEMS Microbiol Lett. 2016 Oct 1;363(19):
pubmed: 27664058
Mol Plant Microbe Interact. 2002 Nov;15(11):1119-27
pubmed: 12423017
Annu Rev Phytopathol. 2014;52:45-68
pubmed: 25001452
Virus Res. 2011 Sep;160(1-2):409-13
pubmed: 21736906
Arch Virol. 2015 Mar;160(3):873-5
pubmed: 25577168
Arch Virol. 2015 Feb;160(2):577-80
pubmed: 25248625
Acta Microbiol Immunol Hung. 2003;50(1):77-94
pubmed: 12793203
PLoS Pathog. 2016 Jun 02;12(6):e1005640
pubmed: 27253323
Arch Virol. 2018 Apr;163(4):1019-1030
pubmed: 29356991
Annu Rev Phytopathol. 2009;47:353-84
pubmed: 19400634
Plant Dis. 2003 Jan;87(1):4-10
pubmed: 30812698
Virology. 2015 May;479-480:356-68
pubmed: 25771805
Arch Virol. 2017 Apr;162(4):1073-1077
pubmed: 28050736
Virology. 2015 Jul;481:151-60
pubmed: 25781585
Front Microbiol. 2017 Sep 06;8:1699
pubmed: 28932216
Appl Environ Microbiol. 2013 Jun;79(12):3684-91
pubmed: 23563943
Nat Rev Microbiol. 2005 Aug;3(8):632-42
pubmed: 16064055
Appl Environ Microbiol. 2002 May;68(5):2529-34
pubmed: 11976130
Arch Virol. 2015 Jun;160(6):1585-8
pubmed: 25854690
J Virol. 2009 Dec;83(24):12801-12
pubmed: 19828620