Genome-wide identification and expression analysis of the StSWEET family genes in potato (Solanum tuberosum L.).
Biotic and abiotic stress
Expression profiles
Gene family
Potato
SWEET
Journal
Genes & genomics
ISSN: 2092-9293
Titre abrégé: Genes Genomics
Pays: Korea (South)
ID NLM: 101481027
Informations de publication
Date de publication:
02 2020
02 2020
Historique:
received:
23
05
2019
accepted:
13
11
2019
pubmed:
30
11
2019
medline:
21
7
2020
entrez:
30
11
2019
Statut:
ppublish
Résumé
The sugar will eventually be exported transporter (SWEET) family is a novel type of membrane-embedded sugar transporter that contains seven transmembrane helices with two MtN3/saliva domains. The SWEET family plays crucial roles in multiple processes, including carbohydrate transportation, development, environmental adaptability and host-pathogen interactions. Although SWEET genes, especially those involved in response to biotic stresses, have been extensively characterized in many plants, they have not yet been studied in potato. The identification of StSWEET genes provides important candidates for further functional analysis and lays the foundation for the production of good quality and high yield potatoes through molecular breeding. In this study, StSWEET genes were identified using a genome-wide search method. A comprehensive analysis of StSWEET family through bioinformatics methods, such as phylogenetic tree, gene structure and promoter prediction analysis. The expression profiles of StSWEET genes in different potato tissues and under P. infestans attack and sugar stress were studied using quantitative real-time polymerase chain reaction (qRT-PCR). Phylogenetic analysis classified 33 StSWEET genes into four groups containing 12, 5, 12 and 4 genes. Furthermore, the gene structures and conserved motifs found that the StSWEET genes are very conservative during evolution. The chromosomal localization pattern showed that the distribution and density of the StSWEETs on 10 potato chromosomes were uneven and basically clustered. Predictive promoter analysis indicated that StSWEET proteins are associated with cell growth, development, secondary metabolism, and response to biotic and abiotic stresses. Finally, the expression patterns of the StSWEET genes in different tissues and the induction of P. infestans and the process of the sugar stress were investigated to obtain the tissue-specific and stress-responsive candidates. This study systematically identifies the SWEET gene family in potato at the genome-wide level, providing important candidates for further functional analysis and contributing to a better understanding of the molecular basis of development and tolerance in potato.
Sections du résumé
BACKGROUND
The sugar will eventually be exported transporter (SWEET) family is a novel type of membrane-embedded sugar transporter that contains seven transmembrane helices with two MtN3/saliva domains. The SWEET family plays crucial roles in multiple processes, including carbohydrate transportation, development, environmental adaptability and host-pathogen interactions. Although SWEET genes, especially those involved in response to biotic stresses, have been extensively characterized in many plants, they have not yet been studied in potato.
OBJECTIVE
The identification of StSWEET genes provides important candidates for further functional analysis and lays the foundation for the production of good quality and high yield potatoes through molecular breeding.
METHODS
In this study, StSWEET genes were identified using a genome-wide search method. A comprehensive analysis of StSWEET family through bioinformatics methods, such as phylogenetic tree, gene structure and promoter prediction analysis. The expression profiles of StSWEET genes in different potato tissues and under P. infestans attack and sugar stress were studied using quantitative real-time polymerase chain reaction (qRT-PCR).
RESULTS
Phylogenetic analysis classified 33 StSWEET genes into four groups containing 12, 5, 12 and 4 genes. Furthermore, the gene structures and conserved motifs found that the StSWEET genes are very conservative during evolution. The chromosomal localization pattern showed that the distribution and density of the StSWEETs on 10 potato chromosomes were uneven and basically clustered. Predictive promoter analysis indicated that StSWEET proteins are associated with cell growth, development, secondary metabolism, and response to biotic and abiotic stresses. Finally, the expression patterns of the StSWEET genes in different tissues and the induction of P. infestans and the process of the sugar stress were investigated to obtain the tissue-specific and stress-responsive candidates.
CONCLUSION
This study systematically identifies the SWEET gene family in potato at the genome-wide level, providing important candidates for further functional analysis and contributing to a better understanding of the molecular basis of development and tolerance in potato.
Identifiants
pubmed: 31782074
doi: 10.1007/s13258-019-00890-y
pii: 10.1007/s13258-019-00890-y
doi:
Substances chimiques
Monosaccharide Transport Proteins
0
Plant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
135-153Références
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