From proof of concept to the routine use of an automated and robust multi-dimensional liquid chromatography mass spectrometry workflow applied for the charge variant characterization of therapeutic antibodies.
Automation
Charge variants
Monoclonal antibodies
Multi-dimensional chromatography
PTM
Journal
Journal of chromatography. A
ISSN: 1873-3778
Titre abrégé: J Chromatogr A
Pays: Netherlands
ID NLM: 9318488
Informations de publication
Date de publication:
29 Mar 2020
29 Mar 2020
Historique:
received:
04
09
2019
revised:
05
11
2019
accepted:
26
11
2019
pubmed:
5
12
2019
medline:
12
5
2020
entrez:
5
12
2019
Statut:
ppublish
Résumé
The identification and quantification of post-translational modifications (PTMs) is a crucial step required during the development of therapeutic proteins. In particular, the characterization of charge variants separated by cation exchange chromatography (CEX) is a tedious process commonly performed with an off-line manual fraction collection followed by peptide mapping. To improve the efficiency of this time-consuming approach, a generic on-line multi-dimensional LC/MS approach was developed for the characterization of various monoclonal antibody (mAb) isotypes and a bi-specific antibody (BsAb). Fractions collected from
Identifiants
pubmed: 31796250
pii: S0021-9673(19)31172-0
doi: 10.1016/j.chroma.2019.460740
pii:
doi:
Substances chimiques
Antibodies, Monoclonal
0
Peptides
0
Asparagine
7006-34-0
Trypsin
EC 3.4.21.4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
460740Informations de copyright
Copyright © 2019. Published by Elsevier B.V.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.