Characterization of three GH35 β-galactosidases, enzymes able to shave galactosyl residues linked to rhamnogalacturonan in pectin, from Penicillium chrysogenum 31B.
Arabinogalactan
Glycoside hydrolase family 35
Pectin
Penicillium chrysogenum
Rhamnogalacturonan-I
β-Galactosidase
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Feb 2020
Feb 2020
Historique:
received:
23
10
2019
accepted:
03
12
2019
revised:
24
11
2019
pubmed:
20
12
2019
medline:
3
10
2020
entrez:
20
12
2019
Statut:
ppublish
Résumé
Three recombinant β-galactosidases (BGALs; PcBGAL35A, PcBGAL35B, and PcGALX35C) belonging to the glycoside hydrolase (GH) family 35 derived from Penicillium chrysogenum 31B were expressed using Pichia pastoris and characterized. PcBGAL35A showed a unique substrate specificity that has not been reported so far. Based on the results of enzymological tests and
Identifiants
pubmed: 31853563
doi: 10.1007/s00253-019-10299-y
pii: 10.1007/s00253-019-10299-y
doi:
Substances chimiques
rhamnogalacturonan I
0
Pectins
89NA02M4RX
beta-Galactosidase
EC 3.2.1.23
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1135-1148Subventions
Organisme : Japan Society for the Promotion of Science KAKENHI
ID : 25450135