Characterization of a novel cotton MYB gene, GhMYB108-like responsive to abiotic stresses.
Amino Acid Sequence
Base Sequence
Droughts
Gene Expression Regulation, Plant
/ drug effects
Gossypium
/ genetics
Phylogeny
Phytochrome
/ pharmacology
Plant Proteins
/ classification
Plants, Genetically Modified
Proto-Oncogene Proteins c-myb
/ classification
Regulatory Elements, Transcriptional
/ genetics
Salinity
Sequence Homology, Amino Acid
Sodium Chloride
/ pharmacology
Stress, Physiological
Bioinformatics
Cotton
Drought
GhMYB108-like
Salinity
Journal
Molecular biology reports
ISSN: 1573-4978
Titre abrégé: Mol Biol Rep
Pays: Netherlands
ID NLM: 0403234
Informations de publication
Date de publication:
Mar 2020
Mar 2020
Historique:
received:
03
10
2019
accepted:
02
01
2020
pubmed:
15
1
2020
medline:
21
10
2020
entrez:
15
1
2020
Statut:
ppublish
Résumé
Transcriptional factors are the major regulators of plant signaling pathways in response to environmental stresses i.e., drought, salinity and cold. Hereby, the GhMYB108-like was characterized to determine whether it regulate these stresses. The GhMYB108-like cDNA consisted of 1107 base pairs (bp) with 807 open reading frame encoded a protein of 268 amino acids. Its isoelectric point and molecular weight are 5.51 and 30.3 kDa respectively. Phylogenetic analysis and online databases revealed that GhMYB108-like proteins are closely related with the Arabidopsis thaliana MYB2. Important cis-elements were detected in the promotor region of GhMYB108-like responding to stresses and phytohormones. The 3D structure of GhMYB108-like protein has been predicted. In addition, various physico-chemical properties of GhMYB108-like have been determined. Subcellular localization confirmed that GhMYB108-like are nuclear localized protein. Quantitative expression analysis showed that polyethylene glycol and salt treatments significantly induced the expression of GhMYB108-like. Overall, our findings suggest that GhMYB108-like is an important gene that would plays important regulatory role in response to drought and salt stresses.
Identifiants
pubmed: 31933260
doi: 10.1007/s11033-020-05244-6
pii: 10.1007/s11033-020-05244-6
doi:
Substances chimiques
Plant Proteins
0
Proto-Oncogene Proteins c-myb
0
Phytochrome
11121-56-5
Sodium Chloride
451W47IQ8X
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1573-1581Subventions
Organisme : National Basic Research Program of China (973 Program)
ID : 2016YFD0101006
Références
Ullah A, Heng S, Munis MFH, Fahad S, Yang X (2015) Phytoremediation of heavy metals assisted by plant growth promoting (PGP) bacteria: a review. Environ Exp Bot 117:28–40
doi: 10.1016/j.envexpbot.2015.05.001
Yu LH, Wu SJ, Peng YS et al (2016) Arabidopsis EDT1/HDG11 improves drought and salt tolerance in cotton and poplar and increases cotton yield in the field. Plant Biotechnol J 14:72–84
doi: 10.1111/pbi.12358
pubmed: 25879154
pmcid: 25879154
Ullah A, Nisar M, Ali H, Hazrat A et al (2019) Drought tolerance improvement in plants: an endophytic bacterial approach. Appl Microbiol Biotechnol 103:7385–7397
doi: 10.1007/s00253-019-10045-4
pubmed: 31375881
pmcid: 31375881
Ullah A, Akbar A, Luo Q, Khan AH, Manghwar H, Shaban M, Yang X (2019) Microbiome diversity in cotton rhizosphere under normal and drought conditions. Microb Ecol 77:429–439
doi: 10.1007/s00248-018-1260-7
pubmed: 30196314
pmcid: 30196314
Comas LH, Becker SR, Cruz VMV, Byrne PF, Dierig DA (2013) Root traits contributing to plant productivity under drought. Front Plant Sci 4:442
doi: 10.3389/fpls.2013.00442
pubmed: 24204374
pmcid: 24204374
Kumar M, Choi JY, Kumari N, Pareek A, Kim SR (2015) Molecular breeding in Brassica for salt tolerance: importance of microsatellite (SSR) markers for molecular breeding in Brassica. Front Plant Sci 6:688
pubmed: 26388887
pmcid: 26388887
Zhang F, Li S, Yang S, Wang L, Guo W (2015) Overexpression of a cotton annexin gene, GhAnn1, enhances drought and salt stress tolerance in transgenic cotton. Plant Mol Biol 87:47–67
doi: 10.1007/s11103-014-0260-3
pubmed: 25330941
pmcid: 25330941
Ullah A, Sun H, Yang X, Zhang X (2017) Drought coping strategies in cotton: increased crop per drop. Plant Biotechnol J 15:281–284
doi: 10.1111/pbi.12688
Dawn news (2016) http://www.dawn.com/news/1240448
Zhang H, Li Y, Zhu JK (2018) Developing naturally stress-resistant crops for a sustainable agriculture. Nat Plants 4:989–996
doi: 10.1038/s41477-018-0309-4
pubmed: 30478360
pmcid: 30478360
Shaban M, Ahmed MM, Sun H, Ullah A, Zhu L (2018) Genome-wide identification of lipoxygenase gene family in cotton and functional characterization in response to abiotic stresses. BMC Genom 19:599
doi: 10.1186/s12864-018-4985-2
Ullah A, Sun H, Yang X, Zhang X (2017) A novel cotton WRKY-gene, GhWRKY6-like, improves salt tolerance by activating the ABA signalling pathway and scavenging of reactive oxygen species. Physiol Plant 162:439–454
doi: 10.1111/ppl.12651
pubmed: 29027659
pmcid: 29027659
Ambawat S, Sharma P, Yadav NR, Yadav RC (2013) MYB transcription factor genes as regulators for plant responses: an overview. Physiol Mol Biol Plants 19:307–321
doi: 10.1007/s12298-013-0179-1
pubmed: 24431500
pmcid: 24431500
Chen T, Li W, Hu X, Guo J, Liu A, Zhang B (2015) A cotton MYB transcription factor, GbMYB5, is positively involved in plant adaptive response to drought stress. Plant Cell Physiol 56:917–929
doi: 10.1093/pcp/pcv019
pubmed: 25657343
pmcid: 25657343
Baldoni E, Genga A, Cominelli E (2015) Plant MYB transcription factors: their role in drought response mechanisms. Int J Mol Sci 16:15811–15851
doi: 10.3390/ijms160715811
pubmed: 26184177
pmcid: 26184177
Xiong H, Li J, Liu P, Duan J, Zhao Y, Guo X, Li Y, Zhang H, Ali J, Li Z (2014) Overexpression of OsMYB48-1, a novel MYB-Related transcription factor, enhances drought and salinity tolerance in rice. PLoS ONE 9:e92913
doi: 10.1371/journal.pone.0092913
pubmed: 3965499
pmcid: 3965499
Gao F, Zhou J, Deng RY, Zhao HX, Li CL, Chen H, Suzuki T, Park S, Wu Q (2017) Overexpression of a tartary buckwheat R2R3-MYB transcription factor gene, FtMYB9, enhances tolerance to drought and salt stresses in transgenic Arabidopsis. J Plant Physiol 214:81–90
doi: 10.1016/j.jplph.2017.04.007
Li K, Xing C, Yao Z, Huang X (2017) PbrMYB21, a novel MYB protein of Pyrus betulaefolia, functions in drought tolerance and modulates polyamine levels by regulating arginine decarboxylase gene. Plant Biotechnol J 15:1186–1203
doi: 10.1111/pbi.12708
pubmed: 5552480
pmcid: 5552480
Jin F, Hu L, Yuan D, Xu J, Gao W, He L, Yang X, Zhang X (2014) Comparative transcriptome analysis between somatic embryos (SEs) and zygotic embryos in cotton: evidence for stress response functions in SE development. Plant Biotechnol J 12:161–173
doi: 10.1111/pbi.12123
Kokkirala VR, Yonggang P, Abbagani S, Zhu Z, Umate P (2010) Subcellular localization of proteins of Oryza sativa L. in the model tobacco and tomato plants. Plant Signal Behav 5:1336–1341
doi: 10.4161/psb.5.11.13318
pubmed: 21045556
pmcid: 21045556
Gasteiger E, Hoogland C, Gattiker A, Duvaud SE, Wilkins MR, Appel RD, Bairoch A (2005) Protein identification and analysis tools on the ExPASy server. In: The proteomics protocols handbook. Humana Press, Totowa
doi: 10.1385/1-59259-890-0:571
Arnold L, Bordoli J, Kopp T, Schwede (2006) The SWISS-MODEL workspace: a web-based environment for protein structure homology modelling. Bioinformatics 22:195–201
doi: 10.1093/bioinformatics/bti770
pubmed: 16301204
pmcid: 16301204
Laskowski RA, Rullmann JA, MacArthur MW, Kaptein R, Thornton JM (1996) AQUA and PROCHECK-NMR: programs for checking the quality of protein structures solved by NMR. J Biomol NMR 8:477–486
doi: 10.1007/BF00228148
pubmed: 9008363
pmcid: 9008363
Wiederstein M, Sippl MJ (2007) ProSA-web: interactive web service for the recognition of errors in three-dimensional structures of proteins. Nucleic Acids Res 35:407–410
doi: 10.1093/nar/gkm290
Benkert P, Kunzli M, Schwede T (2009) QMEAN server for protein model quality estimation. Nucleic Acids Res 37:510–514
doi: 10.1093/nar/gkp322
Colovos C, Yeates TO (1993) Verification of protein structures: patterns of nonbonded atomic interactions. Protein Sci 2:1511–1519
doi: 10.1002/pro.5560020916
pubmed: 8401235
pmcid: 8401235
Liithy R, Bowie JU, Eisenber DM (1992) Assessment of protein models with three-dimensional profiles. Nature 356:83–85
doi: 10.1038/356083a0
Ul Qamar MT, Khan MS (2017) A novel structural and functional insight into chloroplast-encoded central subunit of dark-operated protochlorophyllide oxidoreductase (DPOR) of plants. Pak J Agric Sci 54:395–406
Xu Z, Li J, Guo X, Jin S, Zhang X (2016) Metabolic engineering of cottonseed oil biosynthesis pathway via RNA interference. Sci Rep 6:33342
doi: 10.1038/srep33342
pubmed: 27620452
pmcid: 27620452
Schmittgen TD, Livak KJ (2008) Analyzing real-time PCR data by the comparative CT method. Nat Protoc 3:1101–1108
doi: 10.1038/nprot.2008.73
pubmed: 18546601
Hooft RW, Sander C, Vriend G (1997) Objectively judging the quality of a protein structure from a Ramachandran plot. Comput Appl Biosci 13:425–430
pubmed: 9283757
pmcid: 9283757
Agarwal PK, Shukla PS, Gupta K, Jha B (2013) Bioengineering for salinity tolerance in plants: state of the art. Mol Biotechnol 54:102–123
doi: 10.1007/s12033-012-9538-3
pubmed: 22539206
pmcid: 22539206
Gray SB, Brady SM (2016) Plant developmental responses to climate change. Dev Biol 419:64–77
doi: 10.1016/j.ydbio.2016.07.023
pubmed: 27521050
pmcid: 27521050
Yamaguchi-Shinozaki K, Urao T, Shinozaki K (1995) Regulation of genes that are induced by drought stress in Arabidopsis thaliana. J Plant Res 108:127–136
doi: 10.1007/BF02344316
Wittkopp PJ, Kalay G (2012) Cis-regulatory elements: molecular mechanisms and evolutionary processes underlying divergence. Nat Rev Genet 13:59–69
doi: 10.1038/nrg3095
Ullah A, Manghwar H, Shaban M, Khan AH, Akbar A, Ali U, Ali E, Fahad S (2018) Phytohormones enhanced drought tolerance in plants: a coping strategy. Env Sci Pollut Res 25:33103–33118
doi: 10.1007/s11356-018-3364-5
Zhou L, Wang NN, Gong SY, Lu R, Li Y, Li XB (2015) Overexpression of a cotton (Gossypium hirsutum) WRKY gene, GhWRKY34, in Arabidopsis enhances salt-tolerance of the transgenic plants. Plant Physiol Biochem 96:311–320
doi: 10.1016/j.plaphy.2015.08.016
pubmed: 26332661
pmcid: 26332661
Xie C, Zhang R, Qu Y, Miao Z, Zhang Y, Shen X, Wang T, Dong J (2012) Overexpression of MtCAS31 enhances drought tolerance in transgenic Arabidopsis by reducing stomatal density. New Phytol 195:124–135
doi: 10.1111/j.1469-8137.2012.04136.x
pubmed: 22510066
pmcid: 22510066
Ching T, Himmelstein DS, Beaulieu-Jones BK, Kalinin AA, Do BT, Way GP, Xie W (2018) Opportunities and obstacles for deep learning in biology and medicine. J R Soc Interface 15:20170387
doi: 10.1098/rsif.2017.0387
pubmed: 29618526
pmcid: 29618526