Enzymatic Platforms for Sensitive Neurotransmitter Detection.
Biosensing Techniques
Catalysis
Dopamine
/ urine
Electrochemical Techniques
Electrodes
Enzymes, Immobilized
/ metabolism
Gold
/ chemistry
Horseradish Peroxidase
/ metabolism
Hydrogen-Ion Concentration
Laccase
/ metabolism
Limit of Detection
Microscopy, Atomic Force
Neurotransmitter Agents
/ analysis
Oxidation-Reduction
Platinum
/ chemistry
Polymers
/ chemistry
Serotonin
/ urine
Silicon Compounds
/ chemistry
biosensor
dopamine
horseradish peroxidase
laccase
polymer
serotonin
Journal
Sensors (Basel, Switzerland)
ISSN: 1424-8220
Titre abrégé: Sensors (Basel)
Pays: Switzerland
ID NLM: 101204366
Informations de publication
Date de publication:
11 Jan 2020
11 Jan 2020
Historique:
received:
13
11
2019
revised:
09
12
2019
accepted:
17
12
2019
entrez:
17
1
2020
pubmed:
17
1
2020
medline:
26
9
2020
Statut:
epublish
Résumé
A convenient electrochemical sensing pathway was investigated for neurotransmitter detection based on newly synthesized silole derivatives and laccase/horseradish-peroxidase-modified platinum (Pt)/gold (Au) electrodes. The miniature neurotransmitter's biosensors were designed and constructed via the immobilization of laccase in an electroactive layer of the Pt electrode coated with poly(2,6-bis(3,4-ethylenedioxythiophene)-4-methyl-4-octyl-dithienosilole) and laccase for serotonin (5-HT) detection, and a Au electrode modified with the electroconducting polymer poly(2,6-bis(selenophen-2-yl)-4-methyl-4-octyl-dithienosilole), along with horseradish peroxidase (HRP), for dopamine (DA) monitoring. These sensing arrangements utilized the catalytic oxidation of neurotransmitters to reactive quinone derivatives (the oxidation process was provided in the enzymes' presence). Under the optimized conditions, the analytical performance demonstrated a convenient degree of sensitivity: 0.0369 and 0.0256 μA mM
Identifiants
pubmed: 31940833
pii: s20020423
doi: 10.3390/s20020423
pmc: PMC7014284
pii:
doi:
Substances chimiques
Enzymes, Immobilized
0
Neurotransmitter Agents
0
Polymers
0
Silicon Compounds
0
Serotonin
333DO1RDJY
Platinum
49DFR088MY
Gold
7440-57-5
Laccase
EC 1.10.3.2
Horseradish Peroxidase
EC 1.11.1.-
Dopamine
VTD58H1Z2X
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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