Chimeric LuxR Transcription Factors Rewire Natural Product Regulation.
LuxRs
gene expression
natural products
quorum sensing
transcriptional activation
Journal
Angewandte Chemie (International ed. in English)
ISSN: 1521-3773
Titre abrégé: Angew Chem Int Ed Engl
Pays: Germany
ID NLM: 0370543
Informations de publication
Date de publication:
06 04 2020
06 04 2020
Historique:
received:
13
11
2019
revised:
12
12
2019
pubmed:
17
1
2020
medline:
18
3
2021
entrez:
17
1
2020
Statut:
ppublish
Résumé
LuxR-type transcriptional activator proteins frequently regulate the expression of biosynthetic gene clusters (BGCs). With only a fraction of bacterial BGCs being expressed under standard culturing conditions, modulation of LuxRs would provide a powerful approach to activate silent clusters. We show that by exploiting the modular nature of LuxR proteins, it is possible to construct functional chimeric LuxRs, which enables both the rewiring of quorum sensing systems and the activation of silent BGCs. Importantly, our strategy allowed us to identify the novel natural product pseudomonol from a bacterium of the genus Pseudomonas.
Identifiants
pubmed: 31943579
doi: 10.1002/anie.201914449
pmc: PMC7187462
doi:
Substances chimiques
Biological Products
0
Recombinant Proteins
0
Repressor Proteins
0
Trans-Activators
0
LuxR autoinducer binding proteins
115038-68-1
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
6192-6195Informations de copyright
© 2020 The Authors. Published by Wiley-VCH Verlag GmbH & Co. KGaA.
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