Nitrate and Ammonium Affect the Overall Maize Response to Nitrogen Availability by Triggering Specific and Common Transcriptional Signatures in Roots.
RNA-Seq
amino acids
ammonium
gene expression
maize
nitrate
plant development
root
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
20 Jan 2020
20 Jan 2020
Historique:
received:
16
12
2019
revised:
13
01
2020
accepted:
16
01
2020
entrez:
24
1
2020
pubmed:
24
1
2020
medline:
3
11
2020
Statut:
epublish
Résumé
Nitrogen (N) is an essential macronutrient for crops. Plants have developed several responses to N fluctuations, thus optimizing the root architecture in response to N availability. Nitrate and ammonium are the main inorganic N forms taken up by plants, and act as both nutrients and signals, affecting gene expression and plant development. In this study, RNA-sequencing was applied to gain comprehensive information on the pathways underlying the response of maize root, pre-treated in an N-deprived solution, to the provision of nitrate or ammonium. The analysis of the transcriptome shows that nitrate and ammonium regulate overlapping and distinct pathways, thus leading to different responses. Ammonium activates the response to stress, while nitrate acts as a negative regulator of transmembrane transport. Both the N-source repress genes related to the cytoskeleton and reactive oxygen species detoxification. Moreover, the presence of ammonium induces the accumulation of anthocyanins, while also reducing biomass and chlorophyll and flavonoids accumulation. Furthermore, the later physiological effects of these nutrients were evaluated through the assessment of shoot and root growth, leaf pigment content and the amino acid concentrations in root and shoot, confirming the existence of common and distinct features in response to the two nitrogen forms.
Identifiants
pubmed: 31968691
pii: ijms21020686
doi: 10.3390/ijms21020686
pmc: PMC7013554
pii:
doi:
Substances chimiques
Amino Acids
0
Ammonium Compounds
0
Nitrates
0
Nitrogen
N762921K75
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Università degli Studi di Padova
ID : DOR: 2018-2019
Organisme : Fondazione Cassa di Risparmio di Padova e Rovigo
ID : CARIPARO 2015
Organisme : Università degli Studi di Padova
ID : BIRD 198379
Déclaration de conflit d'intérêts
The authors declare no conflict of interest.
Références
Science. 2018 Sep 14;361(6407):1112-1115
pubmed: 30213912
Mol Plant. 2016 Jun 6;9(6):837-56
pubmed: 27212387
Plant Physiol. 2014 Oct;166(2):509-17
pubmed: 25082891
Plant J. 2004 Mar;37(6):914-39
pubmed: 14996223
Plant Cell. 2010 Nov;22(11):3621-33
pubmed: 21119058
Front Plant Sci. 2016 May 24;7:699
pubmed: 27252716
ScientificWorldJournal. 2015;2015:807560
pubmed: 25879071
Ann Bot. 2017 Mar 1;119(5):703-709
pubmed: 27594647
Front Plant Sci. 2016 Feb 01;7:9
pubmed: 26870047
J Integr Plant Biol. 2014 Nov;56(11):1080-94
pubmed: 24805158
Annu Rev Plant Physiol Plant Mol Biol. 1998 Jun;49:249-279
pubmed: 15012235
Biol Res. 2019 Jul 29;52(1):39
pubmed: 31358053
Food Chem. 2014 Jan 1;142:334-41
pubmed: 24001850
Mol Plant Pathol. 2003 May 1;4(3):203-10
pubmed: 20569380
Amino Acids. 2010 Oct;39(4):917-31
pubmed: 20140469
J Exp Bot. 2017 May 1;68(10):2501-2512
pubmed: 28007951
Nat Biotechnol. 2013 Jan;31(1):46-53
pubmed: 23222703
Physiol Plant. 2012 Nov;146(3):251-60
pubmed: 22568678
Plant Physiol. 2004 Sep;136(1):2483-99
pubmed: 15375205
Plant Physiol. 2003 Jun;132(2):556-67
pubmed: 12805587
Plant Mol Biol. 2016 Aug;91(6):599-606
pubmed: 27003907
Plant Physiol. 2013 Sep;163(1):161-79
pubmed: 23852440
Nature. 2017 Jun 22;546(7659):524-527
pubmed: 28605751
J Exp Bot. 2014 Oct;65(19):5567-76
pubmed: 24942915
Plant Cell Environ. 2009 Sep;32(9):1211-29
pubmed: 19389052
J Exp Bot. 2013 Jan;64(2):553-68
pubmed: 23230025
Plant Physiol Biochem. 2019 Mar;136:178-187
pubmed: 30685697
Bioinformatics. 2014 Aug 1;30(15):2114-20
pubmed: 24695404
J Plant Physiol. 2016 Aug 1;200:62-75
pubmed: 27340859
Gene. 2015 Jul 15;566(1):95-108
pubmed: 25895479
J Exp Bot. 1991 Feb;42(235):189-96
pubmed: 11537730
Int J Mol Sci. 2018 Sep 10;19(9):
pubmed: 30201905
Sci Rep. 2017 Dec 4;7(1):16885
pubmed: 29203827
Amino Acids. 2006 Mar;30(2):127-42
pubmed: 16547652
Proc Natl Acad Sci U S A. 2008 Mar 25;105(12):4939-44
pubmed: 18344319
BMC Plant Biol. 2015 Apr 03;15:96
pubmed: 25886826
Proc Natl Acad Sci U S A. 2006 Dec 12;103(50):19206-11
pubmed: 17148611
BMC Genomics. 2013 Jan 16;14:3
pubmed: 23324127
Plant Sci. 2016 Jul;248:92-101
pubmed: 27181951
Trends Plant Sci. 2010 Feb;15(2):89-97
pubmed: 20036181
J Exp Bot. 2011 Feb;62(4):1499-509
pubmed: 20926552
J Exp Bot. 2015 Jul;66(13):3699-715
pubmed: 25911739
Genome Biol. 2010;11(12):R123
pubmed: 21182762
Plant Physiol. 2007 Dec;145(4):1735-45
pubmed: 17951451
Plant Cell Physiol. 2019 Sep 1;60(9):2100-2112
pubmed: 31147714
Genes (Basel). 2019 May 22;10(5):
pubmed: 31121949
Science. 2014 Oct 17;346(6207):300-1
pubmed: 25324371
Front Plant Sci. 2016 Feb 01;7:29
pubmed: 26870054
J Exp Bot. 2014 Mar;65(3):779-87
pubmed: 24151303
Physiol Plant. 2019 Jul;166(3):794-811
pubmed: 30238472
J Exp Bot. 2007;58(12):3351-60
pubmed: 17977849
Plant Cell Environ. 2010 Sep;33(9):1486-501
pubmed: 20444219
New Phytol. 2011 Oct;192(2):338-52
pubmed: 21762167
Plant Signal Behav. 2014;9(3):e28290
pubmed: 24613869
Arabidopsis Book. 2011;9:e0141
pubmed: 22303266
Plant Direct. 2018 Apr 11;2(4):e00052
pubmed: 31245718
Int J Mol Sci. 2018 Jul 27;19(8):
pubmed: 30060519
Plant Signal Behav. 2006 Mar;1(2):46-51
pubmed: 19521475
Ann Bot. 2015 Feb;115(3):327-31
pubmed: 25844430
Mol Plant. 2014 Feb;7(2):264-76
pubmed: 24285094
Front Plant Sci. 2017 Aug 08;8:1376
pubmed: 28848580
Genome Biol. 2013 Apr 25;14(4):R36
pubmed: 23618408
J Plant Physiol. 2017 May;212:94-104
pubmed: 28282528
Bioinformatics. 2008 Jul 15;24(14):1650-1
pubmed: 18511468
J Exp Bot. 2014 Jan;65(1):185-200
pubmed: 24220653
Food Energy Secur. 2018 Feb;7(1):e00126
pubmed: 29938110
Proc Natl Acad Sci U S A. 2007 Dec 26;104(52):20684-9
pubmed: 18093933
Mol Plant. 2015 Nov 2;8(11):1563-79
pubmed: 26384576
J Exp Bot. 2014 Mar;65(3):789-98
pubmed: 24532451
J Plant Res. 2017 Sep;130(5):859-871
pubmed: 28451936
Bioinformatics. 2009 Aug 15;25(16):2078-9
pubmed: 19505943
Front Plant Sci. 2016 Jun 03;7:760
pubmed: 27375634
Plant J. 2006 Mar;45(5):775-88
pubmed: 16460511
Trends Plant Sci. 2014 Feb;19(2):107-14
pubmed: 24126103
Front Plant Sci. 2016 Feb 18;7:79
pubmed: 26925066
Plant J. 2006 May;46(4):533-48
pubmed: 16640592
Plant Cell Physiol. 2016 Apr;57(4):707-14
pubmed: 27025887
Front Plant Sci. 2016 Jan 22;6:1269
pubmed: 26834770
Gene. 2015 Jan 25;555(2):305-17
pubmed: 25447912
Curr Opin Plant Biol. 2016 Feb;29:57-63
pubmed: 26724502